Vacuole formation in the endothelium of rat extremity vessels depends on fixation techniques and vessel type

Gábor Raffai, E. Fehér, G. Nádasy, S. Paku, Gábor Pogány, Ferenc Tímár, B. Szende, E. Monos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Applying immersion fixation for electron microscopy, huge clear endothelial membrane-bound vacuoles of 0.1-3 μm diameter were noted in the extremity veins of Sprague-Dawley rats. Histological and electron microscopic histochemical methods were applied to determine whether they were the product of programmed cell death or any other kind of cell damage. Image analyzer was used to measure the total area of the vacuoles in the endothelium cells. Neither lipid content nor acidic phosphatase activity could be identified in the vacuoles. In saphenous and brachial veins, the vacuoles occupied 20.6±2.21% and 18±2.45% of the endothelium, respectively. Venous endothelium of two different strains of rat also contained the vacuoles. No such structures appeared in extremity arteries. Long-term tilting did not influence vacuolization. Using in vivo whole body fixation, only pinocytotic and dense microvesicles, but no vacuoles were noted. In conclusion, the clear vacuolar structures represent neither lipid inclusions nor secondary lysosomes. The method of tissue fixation is critical when venous endothelial vesicles are investigated. It is presumed that the vacuoles originated from intra- or intercellular microstructures, and that in case of the collapsible vein segments, their size is increased under the pathological-hypoxic and low-pressure-conditions of in vitro fixation.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalPathology Research and Practice
Volume200
Issue number1
DOIs
Publication statusPublished - Apr 15 2004

Fingerprint

Vacuoles
Endothelium
Extremities
Veins
Tissue Fixation
Lipids
Saphenous Vein
Immersion
Lysosomes
Phosphoric Monoester Hydrolases
Sprague Dawley Rats
Electron Microscopy
Arm
Cell Death
Arteries
Electrons
Pressure
Membranes

Keywords

  • Endothelial vacuole
  • Extremity vessels
  • Histology artefact
  • Vascular histology
  • Venous endothelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Vacuole formation in the endothelium of rat extremity vessels depends on fixation techniques and vessel type. / Raffai, Gábor; Fehér, E.; Nádasy, G.; Paku, S.; Pogány, Gábor; Tímár, Ferenc; Szende, B.; Monos, E.

In: Pathology Research and Practice, Vol. 200, No. 1, 15.04.2004, p. 41-46.

Research output: Contribution to journalArticle

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