Vaccine potential of a nonflagellated, virulence-plasmid-cured (fliD-, pSEVΔ) mutant of salmonella enteritidis for chickens

Ariel Imre, Ama Szmolka, Ferenc Olasz, Béla Nagy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of these studies was to assess residual virulence and early protective capacity of a negatively markered live attenuated vaccine candidate Salmonella Enteritidis mutant against a highly virulent S. Enteritidis strain using a dayold chicken model. Nonflagellated FliD negative mutants of Salmonella Enteritidis 11 (SE11) with and without the virulence plasmid proved to be sufficiently attenuated (limited invasiveness in vitro/in vivo) without reduced ability to colonise chicken gut. The early protective activity of a nonflagellated, virulence-plasmidcured (fliD-, pSEVΔ) mutant against organ invasion, caecal colonisation and faecal shedding by the highly virulent challenge strain S. Enteritidis 147 NalR proved to be effective and safe. The innate and adaptive immunity was demonstrable during the first four weeks of life, and the serological response was clearly distinguishable from the response induced by the wild parental strain. In conclusion, we provided data for the first time about a virulence-plasmid-cured, nonflagellated mutant of S. Enteritidis to serve as a basis for development of a negatively markered potential live oral vaccine against virulent S. Enteritidis in chicken.

Original languageEnglish
Pages (from-to)285-302
Number of pages18
JournalActa veterinaria Hungarica
Volume63
Issue number3
DOIs
Publication statusPublished - Sep 2015

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Keywords

  • Chicken
  • FliD flagellin
  • Salmonella enteritidis
  • Vaccine
  • Virulence plasmid

ASJC Scopus subject areas

  • veterinary(all)

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