UVB light and 17-β-estradiol have different effects on the mRNA expression of Ro/SSA and La/SSB autoantigens in HaCaT cells

Andrea Szegedi, Beatrix Irinyi, Beáta Bessenyei, Marietta Márka, János Hunyadi, I. Semsei

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antibodies produced against the Ro/SSA and La/SSB autoantigens are not only of diagnostic value but they may even play a role in the pathogenesis of several autoimmune diseases (Sjögren's syndrome, subacute cutaneous lupus erythematosus, neonatal lupus erythematosus and systemic lupus erythematosus). Among other factors, ultraviolet (UV) radiation and also the hormonal milieu are well-known co-factors in the pathogenesis of these autoimmune diseases. The goal of our research was to study the possible alterations in mRNA levels of three different Ro antigens and that of two La species produced by alternative splicing in transformed human keratinocytes (HaCaT cells) after UVB irradiation and after 17-β-estradiol treatment. The polymerase chain reaction technique was used to determine the mRNA levels of the Ro and La species after 24, 48, and 72 h of irradiation. The mRNA levels of calreticulin increased as a function of time after UV irradiation but the mRNA levels of 52 kDa and 60 kDa Ro mRNAs were unaltered. After treating the cells with 17-β-estradiol, there was no change observed in the levels of Ro mRNAs or La exon 1 mRNA, but a gradual decrease was noted in the mRNA levels of La exon 1′. The importance of alterations in the ratio of La exon 1 to exon 1′ is supported by the observations in patients with Sjögren's syndrome, and our results strengthen the notion that the Ro and La antigens participate in the pathogenesis of different autoimmune diseases.

Original languageEnglish
Pages (from-to)275-282
Number of pages8
JournalArchives of Dermatological Research
Volume293
Issue number6
DOIs
Publication statusPublished - Jul 30 2001

Keywords

  • Estradiol
  • La/SSB
  • Ro/SSA
  • UVB light
  • mRNA expression

ASJC Scopus subject areas

  • Dermatology

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