UV-Induced small structural changes in the T7 bacteriophage studied by melting methods

K. Tóth, J. Bolard, G. Rontó, D. Aslanian

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

UV optical absorption and circular dichroism (CD) properties (spectra and melting curves) of T7 bacteriophage were investigated to detect "in situ" structural damages which can be related to the biological inactivation due to UV irradiation. UV doses (0.2-1.2 kJ/m2 at 254 nm) near to the biologically effective minimal dose were applied where the initial genetic damage (∼ 10 events/phage) was observed. The decrease of the melting temperature of the helix-coil transition and the broadening of the transition range indicate the destabilization of the intraphage structure due to the presence of about 0.1-0.6% damaged base concentration.

Original languageEnglish
Pages (from-to)229-239
Number of pages11
JournalBiophysics of Structure and Mechanism
Volume10
Issue number4
DOIs
Publication statusPublished - Dec 1983

Fingerprint

Bacteriophage T7
Freezing
Circular Dichroism
Bacteriophages
Temperature

Keywords

  • Absorption and CD melting
  • Bacteriophage
  • Thermal stability of DNA and nucleoproteins
  • UV irradiation effect

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Medicine(all)

Cite this

UV-Induced small structural changes in the T7 bacteriophage studied by melting methods. / Tóth, K.; Bolard, J.; Rontó, G.; Aslanian, D.

In: Biophysics of Structure and Mechanism, Vol. 10, No. 4, 12.1983, p. 229-239.

Research output: Contribution to journalArticle

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