UV-B induced alteration in purinergic receptors and signaling on HaCaT keratinocytes

Olga Ruzsnavszky, Andrea Telek, M. Gönczi, Attila Balogh, E. Remenyik, L. Csernoch

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Although there are a number of recognized risk factors resulting in cutaneous malignancies, very little is known about the exact mechanism. In keratinocytes different purinergic receptors have been implicated to play essential roles in deciding the fate of the cells through regulating proliferation and differentiation. While P2Y receptors seem to control the former, P2X receptors, among which the P2X 7 receptor is associated with the induction of apoptosis, are likely to be responsible for the latter. Forty mJ/cm 2 UV-B irradiation decreased the number of viable cells as assessed using MTT assay. This irradiation decreased the amount of both P2X 1 and P2Y 2 receptors and essentially destroyed the P2X 7 receptors in surviving cells. Morphology of ATP-induced Ca 2+ transients were altered in irradiated cells compared to control. The amplitude and the rate of rise of the transients were decreased and the return to resting [Ca 2+] i prolonged. This observation is consistent with the finding that in control cells mostly ionotropic, while in irradiated cells mostly metabotropic receptors were underlying the response to ATP. These alterations in the expression pattern of purinergic receptors and in the Ca 2+ transients could explain the observed decreased tendency for ATP-induced apoptosis and possibly contribute to the malignant transformation of keratinocytes.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume105
Issue number1
DOIs
Publication statusPublished - Oct 5 2011

Fingerprint

Purinergic Receptors
Keratinocytes
Adenosine Triphosphate
adenosine triphosphate
Apoptosis
apoptosis
cells
Cell Count
irradiation
Skin
induction
tendencies
Neoplasms

Keywords

  • Keratinocytes
  • Purinoreceptor
  • UV-B

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Biophysics

Cite this

UV-B induced alteration in purinergic receptors and signaling on HaCaT keratinocytes. / Ruzsnavszky, Olga; Telek, Andrea; Gönczi, M.; Balogh, Attila; Remenyik, E.; Csernoch, L.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 105, No. 1, 05.10.2011, p. 113-118.

Research output: Contribution to journalArticle

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