UV absorbance of the human cornea in the 240- to 400-nm range

Lajos Kolozsvári, Antal Nógrádi, Béla Hopp, Zsolt Bor

Research output: Contribution to journalArticle

182 Citations (Scopus)


PURPOSE. To determine the UV absorbance of the corneal layers (epithelium, Bowman layer, stroma) in the 240- to 400-nm range. METHODS. Consecutive slices (100 μm) of human cadaveric corneas were cut, and the UV absorbance of each sample was determined in a scanning spectrophotometer. In some cases the epithelium was scraped off and its absorbance measured separately. RESULTS. The investigation of the UV-B absorption of consecutive corneal slices revealed evidence that UV-B absorption is 1.8 times higher in the anterior 100 μm of the human cornea than in the posterior layers. The UV absorbance of the posterior layers was uniform, showing no further structural dependence. The epithelium and Bowman layer are both effective absorbers of UV-B radiation. CONCLUSIONS. These results suggest that the anterior corneal layers are particularly important in preventing damage by UV-B radiation.

Original languageEnglish
Pages (from-to)2165-2168
Number of pages4
JournalInvestigative Ophthalmology and Visual Science
Issue number7
Publication statusPublished - Jul 9 2002

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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