Utilizing new adamantyl spin traps in studying UV-B-induced oxidative damage of photosystem II

Eva Hideg, Anikó Takátsy, Cecilia P. Sár, Imre Vass, Kálmán Hideg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Two recently synthesized adamantyl nitrone spin traps, α-(4-pyridyl-N-oxide)-(-1-adamantyl)-N-nitrone (POAN) and α-(4-pyridyl)-(1-adamantyl)-N-nitrone (PyAN), have been found to be useful for detecting reactive oxygen species in thylakoid membranes. Both show lower inhibition of photosystem II (PSII) electron transport and higher stability of their radical adducts than those of the widely used corresponding phenyl nitrones. Both spin traps are suitable for detecting hydroxyl radicals in W-B-exposed thylakoid membranes when applied at 800 μM. Moreover, at 2 mM, POAN markedly retards UV-B-induced D1 protein degradation. No such effect of PyAN is observed, and neither trap shows marked protection against UV-B-induced electron-transport inactivation. The different chemical structures of the two traps imply different penetration into the thylakoid membrane, suggesting that UV-B-induced D1 protein degradation is brought about by hydroxyl radicals produced at special locations within the thylakoid membrane. these sites are accessible for POAN but inaccessible for the less membrane-penetrating PyAN.

Original languageEnglish
Pages (from-to)174-179
Number of pages6
JournalJournal of Photochemistry and Photobiology B: Biology
Volume48
Issue number2-3
DOIs
Publication statusPublished - Feb 1 1999

Keywords

  • D1 protein
  • EPR spectroscopy
  • Photosynthesis
  • Reactive oxygen
  • Spin traps
  • UV-B stress

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Utilizing new adamantyl spin traps in studying UV-B-induced oxidative damage of photosystem II'. Together they form a unique fingerprint.

  • Cite this