Utilization of lacrimal urea assay in the monitoring of hemodialysis

Conditions, limitations and lacrimal arginase characterization

A. Farkas, Rita Vámos, Tamás Bajor, Nándor Müllner, Ágnes Lázár, András Hrabá

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The lacrimal urea content was found to be proportional to that of blood, which suggested its possible utilization in the monitoring of hemodialysis as a less invasive method. On the other hand, however, arginase activity was detected in tears, which may influence the urea content independently of blood urea concentration. The feasibility of using lacrimal urea measurement to replace blood urea measurement in the monitoring hemodialysis was also investigated. Blood and tear samples of 35 healthy persons and 43 renal patients undergoing hemodialysis were tested. Tear samples were collected on Schirmer paper strips. After elution the lacrimal urea content was measured by a colorimetric method. The determination of arginase activity was based on the release of urea and ornithine. The correlation between blood and lacrimal urea and arginase was studied by multivariate regression analysis. The lacrimal arginase isoenzyme pattern was investigated by native polyacrylamide gel electrophoresis and Western blotting. The effect of partially isoform-specific inhibitors was also studied. Blood urea levels in blood were significantly higher in the renal patients before dialysis than in the control patients (12.86±0.59 vs. 6.45±0.41mM, p

Original languageEnglish
Pages (from-to)183-192
Number of pages10
JournalExperimental Eye Research
Volume76
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Arginase
Tears
Renal Dialysis
Urea
Kidney
Native Polyacrylamide Gel Electrophoresis
Ornithine
Isoenzymes
Dialysis
Protein Isoforms
Multivariate Analysis
Western Blotting
Regression Analysis

Keywords

  • Arginase isoenzymes
  • Blood
  • Hemodialysis
  • Tear
  • Urea

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Utilization of lacrimal urea assay in the monitoring of hemodialysis : Conditions, limitations and lacrimal arginase characterization. / Farkas, A.; Vámos, Rita; Bajor, Tamás; Müllner, Nándor; Lázár, Ágnes; Hrabá, András.

In: Experimental Eye Research, Vol. 76, No. 2, 01.02.2003, p. 183-192.

Research output: Contribution to journalArticle

Farkas, A. ; Vámos, Rita ; Bajor, Tamás ; Müllner, Nándor ; Lázár, Ágnes ; Hrabá, András. / Utilization of lacrimal urea assay in the monitoring of hemodialysis : Conditions, limitations and lacrimal arginase characterization. In: Experimental Eye Research. 2003 ; Vol. 76, No. 2. pp. 183-192.
@article{ad6a76e339cb41908bc0ef295cbb1aee,
title = "Utilization of lacrimal urea assay in the monitoring of hemodialysis: Conditions, limitations and lacrimal arginase characterization",
abstract = "The lacrimal urea content was found to be proportional to that of blood, which suggested its possible utilization in the monitoring of hemodialysis as a less invasive method. On the other hand, however, arginase activity was detected in tears, which may influence the urea content independently of blood urea concentration. The feasibility of using lacrimal urea measurement to replace blood urea measurement in the monitoring hemodialysis was also investigated. Blood and tear samples of 35 healthy persons and 43 renal patients undergoing hemodialysis were tested. Tear samples were collected on Schirmer paper strips. After elution the lacrimal urea content was measured by a colorimetric method. The determination of arginase activity was based on the release of urea and ornithine. The correlation between blood and lacrimal urea and arginase was studied by multivariate regression analysis. The lacrimal arginase isoenzyme pattern was investigated by native polyacrylamide gel electrophoresis and Western blotting. The effect of partially isoform-specific inhibitors was also studied. Blood urea levels in blood were significantly higher in the renal patients before dialysis than in the control patients (12.86±0.59 vs. 6.45±0.41mM, p",
keywords = "Arginase isoenzymes, Blood, Hemodialysis, Tear, Urea",
author = "A. Farkas and Rita V{\'a}mos and Tam{\'a}s Bajor and N{\'a}ndor M{\"u}llner and {\'A}gnes L{\'a}z{\'a}r and Andr{\'a}s Hrab{\'a}",
year = "2003",
month = "2",
day = "1",
doi = "10.1016/S0014-4835(02)00276-2",
language = "English",
volume = "76",
pages = "183--192",
journal = "Experimental Eye Research",
issn = "0014-4835",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Utilization of lacrimal urea assay in the monitoring of hemodialysis

T2 - Conditions, limitations and lacrimal arginase characterization

AU - Farkas, A.

AU - Vámos, Rita

AU - Bajor, Tamás

AU - Müllner, Nándor

AU - Lázár, Ágnes

AU - Hrabá, András

PY - 2003/2/1

Y1 - 2003/2/1

N2 - The lacrimal urea content was found to be proportional to that of blood, which suggested its possible utilization in the monitoring of hemodialysis as a less invasive method. On the other hand, however, arginase activity was detected in tears, which may influence the urea content independently of blood urea concentration. The feasibility of using lacrimal urea measurement to replace blood urea measurement in the monitoring hemodialysis was also investigated. Blood and tear samples of 35 healthy persons and 43 renal patients undergoing hemodialysis were tested. Tear samples were collected on Schirmer paper strips. After elution the lacrimal urea content was measured by a colorimetric method. The determination of arginase activity was based on the release of urea and ornithine. The correlation between blood and lacrimal urea and arginase was studied by multivariate regression analysis. The lacrimal arginase isoenzyme pattern was investigated by native polyacrylamide gel electrophoresis and Western blotting. The effect of partially isoform-specific inhibitors was also studied. Blood urea levels in blood were significantly higher in the renal patients before dialysis than in the control patients (12.86±0.59 vs. 6.45±0.41mM, p

AB - The lacrimal urea content was found to be proportional to that of blood, which suggested its possible utilization in the monitoring of hemodialysis as a less invasive method. On the other hand, however, arginase activity was detected in tears, which may influence the urea content independently of blood urea concentration. The feasibility of using lacrimal urea measurement to replace blood urea measurement in the monitoring hemodialysis was also investigated. Blood and tear samples of 35 healthy persons and 43 renal patients undergoing hemodialysis were tested. Tear samples were collected on Schirmer paper strips. After elution the lacrimal urea content was measured by a colorimetric method. The determination of arginase activity was based on the release of urea and ornithine. The correlation between blood and lacrimal urea and arginase was studied by multivariate regression analysis. The lacrimal arginase isoenzyme pattern was investigated by native polyacrylamide gel electrophoresis and Western blotting. The effect of partially isoform-specific inhibitors was also studied. Blood urea levels in blood were significantly higher in the renal patients before dialysis than in the control patients (12.86±0.59 vs. 6.45±0.41mM, p

KW - Arginase isoenzymes

KW - Blood

KW - Hemodialysis

KW - Tear

KW - Urea

UR - http://www.scopus.com/inward/record.url?scp=0037332157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037332157&partnerID=8YFLogxK

U2 - 10.1016/S0014-4835(02)00276-2

DO - 10.1016/S0014-4835(02)00276-2

M3 - Article

VL - 76

SP - 183

EP - 192

JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

IS - 2

ER -