Using synthetic biological parts and microbioreactors to explore the protein expression characteristics of escherichia coli

Thomas E. Gorochowski, Eric Van Den Berg, Richard Kerkman, J. Roubos, Roel A L Bovenberg

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Synthetic biology has developed numerous parts for the precise control of protein expression. However, relatively little is known about the burden these place on a host, or their reliability under varying environmental conditions. To address this, we made use of synthetic transcriptional and translational elements to create a combinatorial library of constructs that modulated expression strength of a green fluorescent protein. Combining this library with a microbioreactor platform, we were able to perform a detailed large-scale assessment of transient expression and growth characteristics of two Escherichia coli strains across several temperatures. This revealed significant differences in the robustness of both strains to differing types of protein expression, and a complex response of transcriptional and translational elements to differing temperatures. This study supports the development of reliable synthetic biological systems capable of working across different hosts and environmental contexts. Plasmids developed during this work have been made publicly available to act as a reference set for future research.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalACS Synthetic Biology
Volume3
Issue number3
DOIs
Publication statusPublished - Mar 21 2014

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Escherichia coli
Synthetic Biology
Proteins
Temperature
Biological systems
Green Fluorescent Proteins
Libraries
Plasmids
Growth

Keywords

  • biological parts
  • bioreactor
  • characterization
  • protein expression
  • synthetic biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Biomedical Engineering
  • Medicine(all)

Cite this

Using synthetic biological parts and microbioreactors to explore the protein expression characteristics of escherichia coli. / Gorochowski, Thomas E.; Van Den Berg, Eric; Kerkman, Richard; Roubos, J.; Bovenberg, Roel A L.

In: ACS Synthetic Biology, Vol. 3, No. 3, 21.03.2014, p. 129-139.

Research output: Contribution to journalArticle

Gorochowski, Thomas E. ; Van Den Berg, Eric ; Kerkman, Richard ; Roubos, J. ; Bovenberg, Roel A L. / Using synthetic biological parts and microbioreactors to explore the protein expression characteristics of escherichia coli. In: ACS Synthetic Biology. 2014 ; Vol. 3, No. 3. pp. 129-139.
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