Using polysaccharides and galactomannans as gelling agents in capsule formation of artificial seeds

Seppo Sorvari, O. Toldi, Katriina Ahanen, Tuula Viinamäki, Tuna Hakonen, Risto Tahvonen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Somatic embryos that are produced in cell and tissue culture conditions do not form endosperm and testa around the embryo. For many envisioned applications, delicate embryos need to be covered with an artificial endosperm-testa matrix that allows for respiration, contains nutrients and protectants, and is hard enough but allows the vague embryo to germinate and grow into a plant A number of industrially produced and commonly available compounds were tested to determine their efficacy in supporting the germination of encapsulated somatic embryos of carrot (Daucus carota L.). Most of the combinations of galactomannans and polysaccharides were at least as effective as calcium alginate alone in germinating the embryos. The disappearance of blue color in Lugol staining around embryos indicates that starch might also provide nourishment to embryos, thus serving as an artificial endosperm and a supportive matrix.

Original languageEnglish
Pages (from-to)878-883
Number of pages6
JournalJournal of the American Society for Horticultural Science
Volume122
Issue number6
Publication statusPublished - 1997

Fingerprint

galactomannans
artificial seeds
gelling agents
embryo (plant)
Capsules
Polysaccharides
Seeds
polysaccharides
Embryonic Structures
Endosperm
endosperm
somatic embryos
testa
Daucus carota
calcium alginate
carrots
tissue culture
cell culture
Germination
Starch

Keywords

  • Carrot
  • Somatic embryogenesis
  • Starch

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Using polysaccharides and galactomannans as gelling agents in capsule formation of artificial seeds. / Sorvari, Seppo; Toldi, O.; Ahanen, Katriina; Viinamäki, Tuula; Hakonen, Tuna; Tahvonen, Risto.

In: Journal of the American Society for Horticultural Science, Vol. 122, No. 6, 1997, p. 878-883.

Research output: Contribution to journalArticle

Sorvari, Seppo ; Toldi, O. ; Ahanen, Katriina ; Viinamäki, Tuula ; Hakonen, Tuna ; Tahvonen, Risto. / Using polysaccharides and galactomannans as gelling agents in capsule formation of artificial seeds. In: Journal of the American Society for Horticultural Science. 1997 ; Vol. 122, No. 6. pp. 878-883.
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