Using brain slice cultures of mouse brain to assess the effect of growth factors on differentiation of bone marrow derived stem cells.

András Bratincsák, Anna Lonyai, Tal Shahar, Arne Hansen, Zsuzsanna E. Tóth, Eva Mezey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bone marrow derived stem cells (BMDSCs) have been reported to form neurons and supportive cells in the brain. We describe a technique that combines the simplicity of in vitro studies with many of the advantages of in vivo experiments. We cultured mouse brain slices, deposited GFP-tagged BMDSCs evenly distributed on their surfaces, and then added test factors to the culture medium. Addition of both SDF-1 and EGF resulted in morphological changes of BMDSC and in the induction of islet-1, a marker of neuroepithelial progenitors. We conclude that organotypic tissue culture (OTC) may allow us to detect the effects of exogenous factors on the differentiation of BMDSCs (or any other type of stem cells) in an environment that may resemble the CNS after brain injury. Once such factors have been identified they could be evaluated for tissue regeneration in more complex, whole animal models.

Original languageEnglish
Pages (from-to)124-129
Number of pages6
JournalIdeggyógyászati szemle
Volume60
Issue number3-4
Publication statusPublished - Mar 30 2007

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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