Usefulness of transgenic luminescent bacteria in direct bioautographic investigation of chamomile extracts

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28 Citations (Scopus)

Abstract

Direct bioautography performed with luminescence gene-tagged bacteria enables almost real-time detection of antimicrobial compounds in plant extracts. This method for the detection of chamomile ( Matricaria recutita ) components with antibacterial effect against Bacillus subtilis soil bacteria was more sensitive than commonly used bioautographic visualization by staining with a tetrazolium salt. Some compounds had a strong inhibiting effect only via the bioluminescence measurement. Extraction of antibacterial components of chamomile flowers was most effective with 50% ethanol; slightly lower efficiency was achieved with acetone and methanol, and hexane was least effective. The results were confirmed by using luminescent Pseudomonas syringae pv. maculicola plant pathogen bacteria.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume23
Issue number3
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Chamomile
Bacteria
Matricaria
Tetrazolium Salts
Luminescent Measurements
Bioluminescence
Pseudomonas syringae
Plant Extracts
Hexanes
Pathogens
Bacilli
Acetone
Luminescence
Bacillus subtilis
Methanol
Ethanol
Soil
Visualization
Genes
Staining and Labeling

Keywords

  • Bacillus subtilis
  • Bioautography
  • Bioluminescence
  • Chamomile ( Matricaria recutita )
  • Pseudomonas syringae

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

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abstract = "Direct bioautography performed with luminescence gene-tagged bacteria enables almost real-time detection of antimicrobial compounds in plant extracts. This method for the detection of chamomile ( Matricaria recutita ) components with antibacterial effect against Bacillus subtilis soil bacteria was more sensitive than commonly used bioautographic visualization by staining with a tetrazolium salt. Some compounds had a strong inhibiting effect only via the bioluminescence measurement. Extraction of antibacterial components of chamomile flowers was most effective with 50{\%} ethanol; slightly lower efficiency was achieved with acetone and methanol, and hexane was least effective. The results were confirmed by using luminescent Pseudomonas syringae pv. maculicola plant pathogen bacteria.",
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