Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe

M. F. Anjum, E. Jones, V. Morrison, R. Tozzoli, S. Morabito, I. Toth, B. Nagy, G. Smith, A. Aspan, E. M. Nielsen, P. Fach, S. Herrera-León, M. J. Woodward, R. M. La Ragione

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

SUMMARY The presence of 10 virulence genes was examined using polymerase chain reaction (PCR) in 365 European O157 and non-O157 Escherichia coli isolates associated with verotoxin production. Strain-specific PCR data were analysed using hierarchical clustering. The resulting dendrogram clearly separated O157 from non-O157 strains. The former clustered typical high-risk seropathotype (SPT) A strains from all regions, including Sweden and Spain, which were homogenous by Cramer's V statistic, and strains with less typical O157 features mostly from Hungary. The non-O157 strains divided into a high-risk SPTB harbouring O26, O111 and O103 strains, a group pathogenic to pigs, and a group with few virulence genes other than for verotoxin. The data demonstrate SPT designation and selected PCR separated verotoxigenic E. coli of high and low risk to humans; although more virulence genes or pulsed-field gel electrophoresis will need to be included to separate high-risk strains further for epidemiological tracing.

Original languageEnglish
Pages (from-to)1019-1028
Number of pages10
JournalEpidemiology and Infection
Volume142
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Escherichia coli O157
Virulence
Shiga Toxins
Polymerase Chain Reaction
Genes
Shiga-Toxigenic Escherichia coli
Hungary
Pulsed Field Gel Electrophoresis
Sweden
Spain
Cluster Analysis
Swine
Escherichia coli

Keywords

  • Escherichia coli
  • Europe
  • verotoxigenic
  • virulence

ASJC Scopus subject areas

  • Infectious Diseases
  • Epidemiology

Cite this

Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe. / Anjum, M. F.; Jones, E.; Morrison, V.; Tozzoli, R.; Morabito, S.; Toth, I.; Nagy, B.; Smith, G.; Aspan, A.; Nielsen, E. M.; Fach, P.; Herrera-León, S.; Woodward, M. J.; La Ragione, R. M.

In: Epidemiology and Infection, Vol. 142, No. 5, 2014, p. 1019-1028.

Research output: Contribution to journalArticle

Anjum, MF, Jones, E, Morrison, V, Tozzoli, R, Morabito, S, Toth, I, Nagy, B, Smith, G, Aspan, A, Nielsen, EM, Fach, P, Herrera-León, S, Woodward, MJ & La Ragione, RM 2014, 'Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe', Epidemiology and Infection, vol. 142, no. 5, pp. 1019-1028. https://doi.org/10.1017/S0950268813001635
Anjum, M. F. ; Jones, E. ; Morrison, V. ; Tozzoli, R. ; Morabito, S. ; Toth, I. ; Nagy, B. ; Smith, G. ; Aspan, A. ; Nielsen, E. M. ; Fach, P. ; Herrera-León, S. ; Woodward, M. J. ; La Ragione, R. M. / Use of virulence determinants and seropathotypes to distinguish high- and low-risk Escherichia coli O157 and non-O157 isolates from Europe. In: Epidemiology and Infection. 2014 ; Vol. 142, No. 5. pp. 1019-1028.
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