Use of Uracil Thin Layer for Measuring Biologically Effective UV Dose

Pál Gróf, Sándor Gáspár, Györgyi Rontó

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Dimerization of uracil monomers in a polycrystalline state by UV radiation changes the absorption characteristics of a thin layer of the material. The change in optical density, measured by spectrophotometry in the 250-400 nm range, as a function of the exposure time is evaluated in terms of the biologically effective UV dose. A statistical evaluation of a great number of uracil dosimeters irradiated with a TL01 lamp from Philips establishes the possibility of evaluating the biologically effective UV dose using a uracil dosimeter. Nonlinear regression procedures were introduced to correct the absorption spectra for contributions due to light scattering and to determine the optical density values required to calculate the UV dose expressed in Hu units. Comparison of cumulative daily doses and long-term monitoring measured by the uracil thin-layer dosimeter and a phage T7 dosimeter are given, which allow the determination of conversion factors between various biological dosimeters under different irradiation conditions.

Original languageEnglish
Pages (from-to)800-806
Number of pages7
JournalPhotochemistry and photobiology
Issue number5
Publication statusPublished - Nov 1996


ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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