Use of the kinetic plot method to compare the efficiency and resolution of liquid-phase separation techniques based on different driving forces

Veronika Fekete, Ágnes Fekete, Jeno Fekete, Anuschka Liekens, Philippe Schmitt-Kopplin, Gert Desmet

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Much effort has been devoted to improving the capability of separation techniques. The lower the separation volume and the higher the plate number the better the separation. HPLC, UHPLC, CE, and shear-driven chromatography have different separation characteristics. It is shown how the kinetic plot method, originally developed for pressure-driven LC, can be extended to electrically-driven and shear-driven LC. With this extension, the separation speed of pressure-driven, electrically driven, and shear-driven LC can now be compared on a universal basis, i.e. by comparing the separation efficiency or resolution that can be obtained in a given time. As an illustrative example, the separation of coumarin dyes is considered.

Original languageEnglish
Pages (from-to)440-446
Number of pages7
JournalJournal of Planar Chromatography - Modern TLC
Volume23
Issue number6
DOIs
Publication statusPublished - Dec 1 2010

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Keywords

  • CE
  • Column efficiency
  • Column selectivity
  • HPLC
  • Shear-driven chromatography
  • UHPLC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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