Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi

Z. Kováčiková, E. Piecková, E. Tátrai, Z. Pivovarová, M. Mataušic-Pišl

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

There are several reports about chronic intoxications, allergies or other pulmonary health problems of dwellers (especially babies) and workers from buildings contaminated with microfungi. Material from presented work was collected during the study of indoor fungal colonization of mouldy dwellings and public buildings in Slovakia. The isolated fungi were cultivated for 10 days in liquid media and their productsboth endo- and exometabolites were isolated. The metabolites from four isolates of Stachybotrys chartarum, two of Aspergillus versicolor and one of Penicillium chrysogenum were used. Their effects were studied in vitro on rat alveolar epithelial type 2 cells, from the toxicological point of view one of the most important lung cells type. Type 2 cells were cultured for 20 hours in DMEM and then they were exposed for 24 hours to various concentrations of metabolites. The toxicity was evaluated by staining the cells for alkaline phosphatase which is a marker for this cell type. The toxic effect of metabolites from all isolates of Stachybotrys chartarum was dose dependent, there were differencies in the extent of cytotoxicity. From the tested microorganisms Stachabotrys chartarum and Aspergillus versicolor showed high toxic effects (Stachybotrys higher than Aspergillus), while Penicillium chrysogenum only minor toxic effects in this test.

Original languageEnglish
Title of host publicationWIT Transactions on Biomedicine and Health
Pages79-84
Number of pages6
Volume11
DOIs
Publication statusPublished - 2007
Event4th International Conference on the Impact of Environmental Factors on Health 2007 - , Malta
Duration: Jun 27 2007Jun 29 2007

Other

Other4th International Conference on the Impact of Environmental Factors on Health 2007
CountryMalta
Period6/27/076/29/07

Fingerprint

Stachybotrys
Aspergillus
Poisons
Metabolites
Fungi
Penicillium chrysogenum
Cell
Evaluation
Allergies
Alveolar Epithelial Cells
Slovakia
Lung
Phosphatases
Cytotoxicity
Medical problems
Microorganisms
Toxicology
Alkaline Phosphatase
Toxicity
Rats

Keywords

  • Alveolar epithelial type 2 cells
  • Aspergillus versicolor
  • Metabolites
  • Penicillium chrysogenum
  • Stachybotrys chartarum

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Biomedical Engineering
  • Computer Science Applications
  • Modelling and Simulation

Cite this

Kováčiková, Z., Piecková, E., Tátrai, E., Pivovarová, Z., & Mataušic-Pišl, M. (2007). Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi. In WIT Transactions on Biomedicine and Health (Vol. 11, pp. 79-84) https://doi.org/10.2495/EHR070091

Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi. / Kováčiková, Z.; Piecková, E.; Tátrai, E.; Pivovarová, Z.; Mataušic-Pišl, M.

WIT Transactions on Biomedicine and Health. Vol. 11 2007. p. 79-84.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kováčiková, Z, Piecková, E, Tátrai, E, Pivovarová, Z & Mataušic-Pišl, M 2007, Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi. in WIT Transactions on Biomedicine and Health. vol. 11, pp. 79-84, 4th International Conference on the Impact of Environmental Factors on Health 2007, Malta, 6/27/07. https://doi.org/10.2495/EHR070091
Kováčiková Z, Piecková E, Tátrai E, Pivovarová Z, Mataušic-Pišl M. Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi. In WIT Transactions on Biomedicine and Health. Vol. 11. 2007. p. 79-84 https://doi.org/10.2495/EHR070091
Kováčiková, Z. ; Piecková, E. ; Tátrai, E. ; Pivovarová, Z. ; Mataušic-Pišl, M. / Use of the in vitro model for the evaluation of toxic effects of metabolites produced by fungi. WIT Transactions on Biomedicine and Health. Vol. 11 2007. pp. 79-84
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