Use of the BioArena system for indirect detection of endogenous ozone in spots after TLC or OPLC separation

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Singlet oxygen (1O2)-catalyzed oxidation of water leads to a variety of oxidants (e.g. ozone, O3) in host-parasite relationships as well. It can be detected indirectly in TLC or OPLC zones by use of the simple BioArena system and O3-eliminating molecules (e.g. d-limonene and indigo carmine) in the culture medium. It follows from these new findings that not only formaldehyde but also O3 and related bioreactive compounds may play a crucial role in the mechanism of antibacterial activity of antibiotic-like compounds. The toxic potential of a molecule, however, originates from the ratio of the oxidants produced in the chromatographic spots.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalJournal of Planar Chromatography - Modern TLC
Volume21
Issue number2
DOIs
Publication statusPublished - Apr 1 2008

Keywords

  • Aflatoxin B1
  • BioArena
  • Endogenous ozone
  • Formaldehyde
  • Indigo carmine
  • d-Limonene
  • trans-Resveratrol

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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