Use of spectral mapping technique for the comparison of polymer-coated HPLC supports

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The retention time of some model compounds was determined on various reverse-phases high-performance liquid chromatographic (HPLC) columns (polyethylene-coated silica, alumina, zirconia, and octadecyl-coated silica and alumina). The retention capacity and retention selectivity of the columns was separated by the spectral mapping technique followed by two-dimensional nonlinear mapping. Calculation indicated that both the retention capacity and selectivity of the columns considerably depends on the type of the original uncoated support and on the quality and quantity of the hydrocarbon ligand. The result suggest that spectral mapping technique can be successfully used for the separation and determination of retention capacity and retention selectivity of various HPLC columns. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)115-119
Number of pages5
JournalChemometrics and Intelligent Laboratory Systems
Issue number1
Publication statusPublished - Jan 4 2000



  • HPLC supports
  • Retention capacity
  • Retention selectivity
  • Spectral mapping technique

ASJC Scopus subject areas

  • Analytical Chemistry
  • Software
  • Process Chemistry and Technology
  • Spectroscopy
  • Computer Science Applications

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