The optimum conditions of precipitation of the milk-coagulating enzyme from the fermentation liquors of Endothia parasitica and Mucor pusillus strains were established, and the purity of the enzymes recovered in various ways was investigated by paper electrophoresis. It was found that in the case of Endothia parasitica the highest yields were obtained on applying a terminal concentration of 60% of ammonium sulphate (at pH 2 to 4) or of 70% of ethanol (at pH 4 to 6). The loss of activity was nearly the same in both cases up to a temperature of plus 20 degree C. In the case of Mucor pusillus, a terminal concentration of 60% of ammonium sulphate (at pH 2 to 4) or of 70% of ethanol (at pH 4 to 6) was also found to be the most appropriate.
|Number of pages||6|
|Journal||Periodica Polytechnica Chemical Engineering|
|Publication status||Published - Jan 1 1972|
ASJC Scopus subject areas
- Chemical Engineering(all)