Use of peltier thermoelectric devices to control column temperature in high-performance capillary electrophoresis

R. J. Nelson, A. Paulus, A. S. Cohen, A. Guttman, B. L. Karger

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

A temperature control system for high-performance capillary electrophoresis is described utilizing Peltier thermoelectric devices. The thermoelectric devices heat or cool an alumina block which has a high thermal conductivity for efficient heat removal from the external capillary column wall. The heat dissipation characteristics of this device are compared to natural convection and fan cooling by observing the stability of current with time and the dependence of current on applied field. A linear relationship of electroosmotic flow velocity with increasing field is found, indicative of a constant temperature at the inside wall of the capillary. Also, the change in peak shape of horse heart myoglobin as a function of increasing temperature is shown to demonstrate the importance of temperature control in obtaining optimum efficiency.

Original languageEnglish
Pages (from-to)111-127
Number of pages17
JournalJournal of Chromatography A
Volume480
Issue numberC
DOIs
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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