Use of multistep gradient elution TLC to gradient separation in HPLC

Tibor Cserháti, Esther Forgées, Ágnes Kosa, Gergely Csiktusnadi-Kiss, Manuel Candais

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10 Citations (Scopus)


Silica gel, aluminum oxide, and cellulose plates have been tested for their capacity to separate the color pigments of red wine by both adsorption and reversed-phase TLC (RPTLC). As the color pigments of red wines could not be separated isocratically (some of the pigments remained at the origin if weaker mobile phases were used whereas others moved with the solvent front if eluent strength was increased) the application of multistep gradient development was investigated for separation of the pigments in one development using reversed-phase silica layers and water-methanol mixtures as mobile phases. RPTLC can be successfully used for the separation of the color pigments of red wines. The pigment composition determined by RPTLC can facilitate identification of the origin of the wine and aid discovery of adulteration with synthetic coloring substances. It has been further proved that multistep gradient elution in RPTLC can be used to predict the mobile- phase gradient which can, perhaps with slight modification, be used for the same separation in reversed-phase HPLC.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Issue number1
Publication statusPublished - Jan 1 1998



  • Color pigments
  • Food adulteration
  • Modeling HPLC with TLC
  • Multistep gradient elution
  • Red wine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Cserháti, T., Forgées, E., Kosa, Á., Csiktusnadi-Kiss, G., & Candais, M. (1998). Use of multistep gradient elution TLC to gradient separation in HPLC. Journal of Planar Chromatography - Modern TLC, 11(1), 34-37.