Use of multistep gradient elution TLC to gradient separation in HPLC

T. Cserháti, Esther Forgées, Ágnes Kosa, Gergely Csiktusnadi-Kiss, Manuel Candais

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Silica gel, aluminum oxide, and cellulose plates have been tested for their capacity to separate the color pigments of red wine by both adsorption and reversed-phase TLC (RPTLC). As the color pigments of red wines could not be separated isocratically (some of the pigments remained at the origin if weaker mobile phases were used whereas others moved with the solvent front if eluent strength was increased) the application of multistep gradient development was investigated for separation of the pigments in one development using reversed-phase silica layers and water-methanol mixtures as mobile phases. RPTLC can be successfully used for the separation of the color pigments of red wines. The pigment composition determined by RPTLC can facilitate identification of the origin of the wine and aid discovery of adulteration with synthetic coloring substances. It has been further proved that multistep gradient elution in RPTLC can be used to predict the mobile- phase gradient which can, perhaps with slight modification, be used for the same separation in reversed-phase HPLC.

Original languageEnglish
Pages (from-to)34-37
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume11
Issue number1
Publication statusPublished - Jan 1998

Fingerprint

Wine
Pigments
High Pressure Liquid Chromatography
Color
Aluminum Oxide
Silica Gel
Cellulose
Silicon Dioxide
Adsorption
Methanol
Coloring
Water
Chemical analysis

Keywords

  • Color pigments
  • Food adulteration
  • Modeling HPLC with TLC
  • Multistep gradient elution
  • Red wine

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Cserháti, T., Forgées, E., Kosa, Á., Csiktusnadi-Kiss, G., & Candais, M. (1998). Use of multistep gradient elution TLC to gradient separation in HPLC. Journal of Planar Chromatography - Modern TLC, 11(1), 34-37.

Use of multistep gradient elution TLC to gradient separation in HPLC. / Cserháti, T.; Forgées, Esther; Kosa, Ágnes; Csiktusnadi-Kiss, Gergely; Candais, Manuel.

In: Journal of Planar Chromatography - Modern TLC, Vol. 11, No. 1, 01.1998, p. 34-37.

Research output: Contribution to journalArticle

Cserháti, T, Forgées, E, Kosa, Á, Csiktusnadi-Kiss, G & Candais, M 1998, 'Use of multistep gradient elution TLC to gradient separation in HPLC', Journal of Planar Chromatography - Modern TLC, vol. 11, no. 1, pp. 34-37.
Cserháti, T. ; Forgées, Esther ; Kosa, Ágnes ; Csiktusnadi-Kiss, Gergely ; Candais, Manuel. / Use of multistep gradient elution TLC to gradient separation in HPLC. In: Journal of Planar Chromatography - Modern TLC. 1998 ; Vol. 11, No. 1. pp. 34-37.
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