Use of monoclonal antibodies in formol-paraffin sections - A study of normal and neoplastic haemopoietic tissues

T. Magyarlaki, G. Kelényi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Thirteen monoclonal antibodies (the "VI-series") reactive with B- and T-lymphocytes, monocytes, granulocytes and erythrocytes were tested in sections fixed with formalin, formalin-sublimate or formalin-acetic acid. After fixation and embedding, most of the antigens were not detectable. However, VIE-G 4, a monoclonal antibody selective for glycophorin A, produced a strong reaction with erythroid cells in formalin fixed sections. The binding of the monoclonal antibodies VIM-D 5, VIM-2 and VIM-13 to myeloid, myelomonocytic and monocytic cells became either more intense or was inhibited by preincubation of the sections with pronase, trypsin, papain or neuraminidase. Following enzyme digestion, the monocyte-specific antibody VIM-13 reacted selectively with some of the germinal centre cells.

Original languageEnglish
Pages (from-to)487-492
Number of pages6
JournalVirchows Archiv A Pathological Anatomy and Histopathology
Volume412
Issue number5
DOIs
Publication statusPublished - Sep 1988

Fingerprint

Paraffin
Formaldehyde
Monoclonal Antibodies
Monocytes
Glycophorin
Mercuric Chloride
Pronase
Erythroid Cells
Germinal Center
Papain
Neuraminidase
Granulocytes
Acetic Acid
Trypsin
Digestion
Erythrocytes
T-Lymphocytes
Antigens
Antibodies
Enzymes

Keywords

  • Centroblast reaction
  • Enzyme pretreatment
  • Formol-paraffin sections
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Anatomy

Cite this

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