Use of molecular markers for studying genetic diversity in durum wheat (Triticum durum Desf.)

P. Szücs, A. Juhász, I. Karsai, L. Láng, O. Veisz, Z. Bedő

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Diversity analysis was carried out using RFLP and RAPD markers to reveal differences between T. durum genotypes. In the RAPD analyses, 23 winter T. durum genotypes of different origins were examined (using three T. aestivum and three T. monococcum varieties as reference genotypes). Five of these were also tested using the RFLP method. Polymorphism was demonstrated between the 5 winter T. durum genotypes by 13 of the 47 RFLP probe X restriction endonuclease combinations (28%) and by 8 of the 16 RAPD primers (50%). Similarity values ranging from 0.04 to 0.14 were obtained with RFLP and 0.03 to 0.18 with RAPD. The correlation between the JD values obtained using the two methods was moderate, but not significant (r2=0.42). In the course of divergence analysis of the 23 T. durum genotypes using the RAPD method, 87.5% of the 16 primers revealed polymorphism. In this case, the value of the JD coefficient was between 0.03 and 0.74. The JD coefficients closely correlated to the groupings suggested by the pedigrees. In the present experiments, polymorphism was successfully demonstrated between T. durum genotypes with a relatively narrow genetic basis using RFLP probes and RAPD primers. These results can be utilised in the design of breeding programmes and could help in the development of T. durum crosses aimed at mapping DNA markers linked to various agronomic traits. They also draw attention to the restricted genetic basis of the cultivated T. durum genotypes.

Original languageEnglish
Pages (from-to)25-33
Number of pages9
JournalJournal of Genetics and Breeding
Volume54
Issue number1
Publication statusPublished - Jan 2000

Fingerprint

Triticum turgidum subsp. durum
durum wheat
Triticum
Genotype
Restriction Fragment Length Polymorphisms
genetic variation
genetic markers
restriction fragment length polymorphism
genotype
genetic polymorphism
probes (equipment)
winter
DNA Restriction Enzymes
restriction endonucleases
Pedigree
Genetic Markers
agronomic traits
pedigree
Breeding
methodology

Keywords

  • Durum wheat
  • Genetic variability
  • RAPD
  • RFLP

ASJC Scopus subject areas

  • Horticulture
  • Plant Science
  • Genetics

Cite this

Use of molecular markers for studying genetic diversity in durum wheat (Triticum durum Desf.). / Szücs, P.; Juhász, A.; Karsai, I.; Láng, L.; Veisz, O.; Bedő, Z.

In: Journal of Genetics and Breeding, Vol. 54, No. 1, 01.2000, p. 25-33.

Research output: Contribution to journalArticle

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