Use of hemicellulose hydrolysate for β-glucosidase fermentation

K. Réczey, A. Brumbauer, M. Bollók, Z. S. Szengyel, G. Zacchi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Hydrolysis of cellulose by Trichoderma cellulases often results in a mixture of glucose, cellobiose, and low-mol-wt cellodextrins. Cellobiose is nonfermentable for most yeasts, and therefore it has to be hydrolyzed to glucose by β-glucosidase prior to ethanol fermentation. In the present study, the β-glucosidase production of one Penicillium and three Aspergillus strains, which were previously selected out of 24 strains, was investigated on steam pretreated willow. Both steam-pretreated willow and hemicellulose hydrolysate, released during steam explosion of willow, were used as carbon sources. Reference cultivation runs were performed using prehydrolyzed Solka Floc and glucose. The four strains were compared with Trichoderma reesei regarding sugar consumption and β-glucosidase production. Aspergillus niger and Aspergillus phoenicis proved to be the best enzyme producers on hemicellulose hydrolysate. The maximum β-glucosidas activity, 4.60 IU/mL, was obtained when A. phoenicis was cultivated or the mixture of hemicellulose hydrolysate and steam-pretreated willow. The maximum yield of enzyme activity, 502 IU/g total carbohydrate, was obtained when Aspergillus foetidus was cultivated on the hemicellulose hydrolysate.

Original languageEnglish
Pages (from-to)225-235
Number of pages11
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume70-72
Publication statusPublished - 1998

Fingerprint

Salix
Glucosidases
Aspergillus
Steam
Fermentation
Glucose
Cellobiose
Trichoderma
Cellulases
Aspergillus niger
Explosions
Penicillium
Enzyme activity
Enzymes
Carbohydrates
Cellulose
Sugars
Yeast
Hydrolysis
Ethanol

Keywords

  • β-glucosidase production
  • Aspergillus
  • Hemicellulose hydrolysate of willow
  • Penicillium
  • Trichoderma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

Use of hemicellulose hydrolysate for β-glucosidase fermentation. / Réczey, K.; Brumbauer, A.; Bollók, M.; Szengyel, Z. S.; Zacchi, G.

In: Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology, Vol. 70-72, 1998, p. 225-235.

Research output: Contribution to journalArticle

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