Use of complexing agents for selective separation in high-performance capillary electrophoresis. Chiral resolution via cyclodextrins incorporated within polyacrylamide gel columns

A. Guttman, A. Paulus, A. S. Cohen, N. Grinberg, B. L. Karger

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

The incorporation of a complexing agent within a polyarcylamide gel column provides a general means of manipulating the selectivity of a capillary electrophoresis separation. As an example of this approach, chiral resolution of dansylated amino acids by high-performance capillary electrophoresis has been achieved by adding β-cyclodextrin within the gel matrix. A retention model has been developed which can be used for selectivity optimization. Various parameters, such as concentration of cyclodextrin, addition of methanol to the buffer, and temperature, have been examined in terms of their influence on retention and selectivity. From this study high-performance separations have been developed. Efficiencies as high as 100 000 plates in 15 cm have been achieved.

Original languageEnglish
Pages (from-to)41-53
Number of pages13
JournalJournal of Chromatography A
Volume448
Issue numberC
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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