Use of chromatofocusing for separation of β-lactamases. VIII. Analytical chromatofocusing of chromosomal cephalosporinases from four klebsiella strains

Susanne Gál, Andrea Tar, Magdolna Frommer-Filep, Bela L. Toth-Martinez, Ferenc J. Hernádi, L. Kiss

Research output: Contribution to journalArticle

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Abstract

Although still there are Klebsiella strains which do not harbour plasmids and produce constitutive chromosomal β-lactamases, recently clinical isolates were found in ever increasing numbers carrying mainly TEM-, CARB- and OXA type R-factors. We selected four chromosomal cephalosporinase producing Klebsiella strains to study the pI values of the enzymes and their simultaneous separability from accompanying proteins by chromatofocusing techniques. We compared pI values of the pure and the crude preparations: K. pneumoniae Kl SC 10436: pIpure = 6.4, pIcrude = 6.42; K. aerogenes Kl 1082 E: pIpure = 6.5, pIcrude = 6.5; K. oxytoca 1082 E: pIpure = 6.42, pIcrude = 6.4; K. oxytoca 20: pIpure = 7.62, pIcrude = 7.6. Excellent agreement of the pI values among each other, but occasional differences with those obtained by analytical isoelectrofocusing are attributed to methodological diversities and to the presence of satellite enzymes, known to exist in Klebsiella.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalJournal of Chromatography A
Volume403
Issue numberC
DOIs
Publication statusPublished - 1987

Fingerprint

Klebsiella
R388
Cephalosporinase
Enzymes
Ports and harbors
Plasmids
Satellites
Transmission electron microscopy
Pneumonia
Proteins
Preparation K
Asp(5)-oxytocin

ASJC Scopus subject areas

  • Analytical Chemistry

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Use of chromatofocusing for separation of β-lactamases. VIII. Analytical chromatofocusing of chromosomal cephalosporinases from four klebsiella strains. / Gál, Susanne; Tar, Andrea; Frommer-Filep, Magdolna; Toth-Martinez, Bela L.; Hernádi, Ferenc J.; Kiss, L.

In: Journal of Chromatography A, Vol. 403, No. C, 1987, p. 217-224.

Research output: Contribution to journalArticle

Gál, Susanne ; Tar, Andrea ; Frommer-Filep, Magdolna ; Toth-Martinez, Bela L. ; Hernádi, Ferenc J. ; Kiss, L. / Use of chromatofocusing for separation of β-lactamases. VIII. Analytical chromatofocusing of chromosomal cephalosporinases from four klebsiella strains. In: Journal of Chromatography A. 1987 ; Vol. 403, No. C. pp. 217-224.
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