Meghatározott kémiai összetételu táptalajok használata szarvasmarhapetesejtek mé lyhutése során

Translated title of the contribution: Use of chemically defined media for the vitrification of bovine oocytes

Horváth Gabriella, L. Solti

Research output: Contribution to journalArticle

Abstract

Most media used during oocyte vitrification contains protein sources of animal origin, which may raise epidemiological concerns, and as these compounds are not defined chemically, the outcome of vitrification may vary, as well. The aim of the study was to exclude proteins of animal origin from media and to replace it with chemically defined compounds. Prior to vitrification, the oocytes were pretreated with cholesterol loaded into methyl- ß-cyclodextrin or fetuin. After vitrification, the cholesterol treated group of oocytes resulted in higher cleavage rate and 8-cell-stage rate in comparison to the untreated oocytes or the ones vitrified with the traditional fetal calf serum containing media, but no effect was seen in their further development. The fetuin supplementation before vitrification did not result in any advantage, but the results were comparable to the standard process, during which fetal calf serum was used.

Original languageHungarian
Pages (from-to)227-231
Number of pages5
JournalMagyar Allatorvosok Lapja
Volume130
Issue number4
Publication statusPublished - 2008

Fingerprint

Vitrification
vitrification
Oocytes
oocytes
fetuins
Fetuins
cattle
fetal bovine serum
Cholesterol
cholesterol
animal source protein
cyclodextrins
animal proteins
Cyclodextrins
Serum
Proteins
cells

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Meghatározott kémiai összetételu táptalajok használata szarvasmarhapetesejtek mé lyhutése során. / Gabriella, Horváth; Solti, L.

In: Magyar Allatorvosok Lapja, Vol. 130, No. 4, 2008, p. 227-231.

Research output: Contribution to journalArticle

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