Use-dependent action of antiarrhythmic drugs in frog skeletal muscle and canine cardiac Purkinje fiber

P. Nánási, A. Varró, David A. Lathrop, Miklós Dankó

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

1. Conventional microelectrode techniques were used to study the effect of quinidine (10 μM), lidocaine (20 μM), and verapamil (3-10 μM) on action potential upstroke (V+max) in frog skeletal muscle and dog Purkinje fiber. 2. The frequency-dependent nature of V+max depression induced by these drugs was similar in both preparations, however, quinidine was more potent in skeletal muscle while lidocaine was in Purkinje fibers. 3. In skeletal muscle tetrodotoxin (3 and 15 nM) and low concentrations of antiarrhythmic drugs proportionally reduced the maximum velocity of depolarization and repolarization (V+max and V-max, respectively), whereas V-max was more depressed than V+max by high concentrations (50-200 μM) of antiarrhythmics. Decreases in the overshoot potential were proportional to the V+max block in the case of each drug. 4. These results indicate that therapeutically relevant concentrations of quinidine and lidocaine inhibit skeletal muscle Na+ channels in a use-dependent manner similar to heart, while at higher concentrations the K+ channels may also be blocked. Therapeutic implications of the results are discussed.

Original languageEnglish
Pages (from-to)747-751
Number of pages5
JournalGeneral Pharmacology
Volume21
Issue number5
DOIs
Publication statusPublished - 1990

Fingerprint

Purkinje Fibers
Anti-Arrhythmia Agents
Anura
Quinidine
Canidae
Skeletal Muscle
Lidocaine
Tetrodotoxin
Microelectrodes
Verapamil
Pharmaceutical Preparations
Action Potentials
Dogs

ASJC Scopus subject areas

  • Pharmacology

Cite this

Use-dependent action of antiarrhythmic drugs in frog skeletal muscle and canine cardiac Purkinje fiber. / Nánási, P.; Varró, A.; Lathrop, David A.; Dankó, Miklós.

In: General Pharmacology, Vol. 21, No. 5, 1990, p. 747-751.

Research output: Contribution to journalArticle

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