Urothelial cells produce hydrogen peroxide through the activation of Duox1

Ágnes Donkó, Éva Ruisanchez, Anna Orient, Balázs Enyedi, Réka Kapui, Zalán Péterfi, Xavier De Deken, Zoltán Benyó, M. Geiszt

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) has important messenger and effector functions in the plant and animal kingdom. Phagocytes produce H 2O2 to kill pathogens, and epithelial cells of large airways have also been reported to produce H2O2 for signaling and host defense purposes. In this report, we show for the first time that urothelial cells produce H2O2 in response to a calcium signal. Using a gene-deficient mouse model we also demonstrate that H2O2 is produced by the NADPH oxidase Duox1, which is expressed in the mouse urothelium. In contrast, we found no evidence for the expression of lactoperoxidase, an enzyme that has been shown to cooperate with Duox enzymes. We also found that specific activation of TRPV4 calcium channels elicits a calcium signal and stimulates H2O2 production in urothelial cells. Furthermore, we detected altered pressure responses in the urinary bladders of Duox1 knockout animals. Our results raise the possibility that mechanosensing in epithelial cells involves calcium-dependent H 2O2 production similar to that observed in plants.

Original languageEnglish
Pages (from-to)2040-2048
Number of pages9
JournalFree Radical Biology and Medicine
Volume49
Issue number12
DOIs
Publication statusPublished - Dec 15 2010

Fingerprint

Hydrogen Peroxide
Chemical activation
Calcium
Animals
Epithelial Cells
Lactoperoxidase
Urothelium
NADPH Oxidase
Pathogens
Enzymes
Calcium Channels
Phagocytes
Urinary Bladder
Genes
Pressure

Keywords

  • Duox
  • NADPH oxidase
  • OmniBank®
  • Reactive oxygen species
  • TRPV4
  • Urothelium

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Urothelial cells produce hydrogen peroxide through the activation of Duox1. / Donkó, Ágnes; Ruisanchez, Éva; Orient, Anna; Enyedi, Balázs; Kapui, Réka; Péterfi, Zalán; De Deken, Xavier; Benyó, Zoltán; Geiszt, M.

In: Free Radical Biology and Medicine, Vol. 49, No. 12, 15.12.2010, p. 2040-2048.

Research output: Contribution to journalArticle

Donkó, Á, Ruisanchez, É, Orient, A, Enyedi, B, Kapui, R, Péterfi, Z, De Deken, X, Benyó, Z & Geiszt, M 2010, 'Urothelial cells produce hydrogen peroxide through the activation of Duox1', Free Radical Biology and Medicine, vol. 49, no. 12, pp. 2040-2048. https://doi.org/10.1016/j.freeradbiomed.2010.09.027
Donkó Á, Ruisanchez É, Orient A, Enyedi B, Kapui R, Péterfi Z et al. Urothelial cells produce hydrogen peroxide through the activation of Duox1. Free Radical Biology and Medicine. 2010 Dec 15;49(12):2040-2048. https://doi.org/10.1016/j.freeradbiomed.2010.09.027
Donkó, Ágnes ; Ruisanchez, Éva ; Orient, Anna ; Enyedi, Balázs ; Kapui, Réka ; Péterfi, Zalán ; De Deken, Xavier ; Benyó, Zoltán ; Geiszt, M. / Urothelial cells produce hydrogen peroxide through the activation of Duox1. In: Free Radical Biology and Medicine. 2010 ; Vol. 49, No. 12. pp. 2040-2048.
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