Urokinase-type plasminogen activator in rabbit tears. Comparison with human tears

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Plasminogen activator activity was determined in stimulated normal rabbit tears, and was identified as being due to urokinase using specific antibodies. The activator activity is about 100-fold higher in rabbit tears than in human tears. The molecular weight of the activator was determined by fibrin zymography to be 40-50 kDa. However, a multiband pattern of urokinase-type antigen ranging in molecular weight from 60-70 kDa to 200 kDa was detected by immunoblotting. The 60-70 kDa protein was resistant to reduction. The antigen concentration is more than 100-fold higher than the concentration expected from the activity measurement, indicating that most of the urokinase in rabbit tears is in inactive form, such as in inhibitor or receptor complexes.

Original languageEnglish
Pages (from-to)33-37
Number of pages5
JournalExperimental Eye Research
Volume51
Issue number1
DOIs
Publication statusPublished - 1990

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Urokinase-Type Plasminogen Activator
Tears
Rabbits
Molecular Weight
Antigens
Plasminogen Activators
Fibrin
Immunoblotting
Antibodies
Proteins

Keywords

  • chromogenic method
  • fibrin zymography
  • immunoblotting
  • plasminogen activator
  • rabbit tears

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Urokinase-type plasminogen activator in rabbit tears. Comparison with human tears. / Tőzsér, J.; Berta, A.

In: Experimental Eye Research, Vol. 51, No. 1, 1990, p. 33-37.

Research output: Contribution to journalArticle

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