Urinary steroid profile in early pregnancy after in vitro fertilization

Ildikó Bíró, Anita Bufa, Ferenc Wilhelm, Zoltán Mánfai, F. Kilár, Péter Miklós Gocze

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective. The aim of the study was to compare the levels of urinary steroid metabolites of patients with successful in vitro fertilization and patients who failed to achieve pregnancy. Design. Comparison of urinary steroid profiles prior to oocyte pick-up and three weeks after embryo transfer. Setting. University hospital. Sample. Eleven women in the same age range with pregnancy after in vitro fertilization and eleven women who failed to achieve pregnancy. Methods. The standard "long" protocol was used for ovarian stimulation and intracytoplasmic sperm injection for assisted in vitro fertilization. The steroid metabolites in urine samples collected for 24 h were determined by gas chromatography-mass spectrometry. Main outcome measures. Steroid metabolite levels in urine samples determined in the early pregnancy period. Results. The levels of androsterone, etiocholanolone, pregnanediol, tetrahydro-11- dehydrocorticosterone and tetrahydro-corticosterone were significantly higher (p <0.05) in the urine of women with successful pregnancy three weeks after the embryo transfer, while the levels of tetrahydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and α-cortolone became higher in the group of patients with unsuccessful pregnancy. Conclusions. The production of androgens, progesterone and corticoid steroid metabolites is altered in the early pregnancy period after in vitro fertilization.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalActa Obstetricia et Gynecologica Scandinavica
Volume91
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

Fertilization in Vitro
Steroids
Pregnancy
Tetrahydrocortisol
Embryo Transfer
Urine
Tetrahydrocortisone
Etiocholanolone
Pregnanediol
Androsterone
Intracytoplasmic Sperm Injections
Ovulation Induction
Corticosterone
Gas Chromatography-Mass Spectrometry
Androgens
Oocytes
Progesterone
Adrenal Cortex Hormones
Outcome Assessment (Health Care)

Keywords

  • early pregnancy
  • gas chromatography-mass spectrometry
  • In vitro fertilization
  • urinary steroids

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Urinary steroid profile in early pregnancy after in vitro fertilization. / Bíró, Ildikó; Bufa, Anita; Wilhelm, Ferenc; Mánfai, Zoltán; Kilár, F.; Gocze, Péter Miklós.

In: Acta Obstetricia et Gynecologica Scandinavica, Vol. 91, No. 5, 05.2012, p. 625-629.

Research output: Contribution to journalArticle

Bíró, Ildikó ; Bufa, Anita ; Wilhelm, Ferenc ; Mánfai, Zoltán ; Kilár, F. ; Gocze, Péter Miklós. / Urinary steroid profile in early pregnancy after in vitro fertilization. In: Acta Obstetricia et Gynecologica Scandinavica. 2012 ; Vol. 91, No. 5. pp. 625-629.
@article{10d4b948e6974e8d96a6ac7b8a6b4771,
title = "Urinary steroid profile in early pregnancy after in vitro fertilization",
abstract = "Objective. The aim of the study was to compare the levels of urinary steroid metabolites of patients with successful in vitro fertilization and patients who failed to achieve pregnancy. Design. Comparison of urinary steroid profiles prior to oocyte pick-up and three weeks after embryo transfer. Setting. University hospital. Sample. Eleven women in the same age range with pregnancy after in vitro fertilization and eleven women who failed to achieve pregnancy. Methods. The standard {"}long{"} protocol was used for ovarian stimulation and intracytoplasmic sperm injection for assisted in vitro fertilization. The steroid metabolites in urine samples collected for 24 h were determined by gas chromatography-mass spectrometry. Main outcome measures. Steroid metabolite levels in urine samples determined in the early pregnancy period. Results. The levels of androsterone, etiocholanolone, pregnanediol, tetrahydro-11- dehydrocorticosterone and tetrahydro-corticosterone were significantly higher (p <0.05) in the urine of women with successful pregnancy three weeks after the embryo transfer, while the levels of tetrahydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and α-cortolone became higher in the group of patients with unsuccessful pregnancy. Conclusions. The production of androgens, progesterone and corticoid steroid metabolites is altered in the early pregnancy period after in vitro fertilization.",
keywords = "early pregnancy, gas chromatography-mass spectrometry, In vitro fertilization, urinary steroids",
author = "Ildik{\'o} B{\'i}r{\'o} and Anita Bufa and Ferenc Wilhelm and Zolt{\'a}n M{\'a}nfai and F. Kil{\'a}r and Gocze, {P{\'e}ter Mikl{\'o}s}",
year = "2012",
month = "5",
doi = "10.1111/j.1600-0412.2012.01387.x",
language = "English",
volume = "91",
pages = "625--629",
journal = "Acta Obstetricia et Gynecologica Scandinavica, Supplement",
issn = "0001-6349",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Urinary steroid profile in early pregnancy after in vitro fertilization

AU - Bíró, Ildikó

AU - Bufa, Anita

AU - Wilhelm, Ferenc

AU - Mánfai, Zoltán

AU - Kilár, F.

AU - Gocze, Péter Miklós

PY - 2012/5

Y1 - 2012/5

N2 - Objective. The aim of the study was to compare the levels of urinary steroid metabolites of patients with successful in vitro fertilization and patients who failed to achieve pregnancy. Design. Comparison of urinary steroid profiles prior to oocyte pick-up and three weeks after embryo transfer. Setting. University hospital. Sample. Eleven women in the same age range with pregnancy after in vitro fertilization and eleven women who failed to achieve pregnancy. Methods. The standard "long" protocol was used for ovarian stimulation and intracytoplasmic sperm injection for assisted in vitro fertilization. The steroid metabolites in urine samples collected for 24 h were determined by gas chromatography-mass spectrometry. Main outcome measures. Steroid metabolite levels in urine samples determined in the early pregnancy period. Results. The levels of androsterone, etiocholanolone, pregnanediol, tetrahydro-11- dehydrocorticosterone and tetrahydro-corticosterone were significantly higher (p <0.05) in the urine of women with successful pregnancy three weeks after the embryo transfer, while the levels of tetrahydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and α-cortolone became higher in the group of patients with unsuccessful pregnancy. Conclusions. The production of androgens, progesterone and corticoid steroid metabolites is altered in the early pregnancy period after in vitro fertilization.

AB - Objective. The aim of the study was to compare the levels of urinary steroid metabolites of patients with successful in vitro fertilization and patients who failed to achieve pregnancy. Design. Comparison of urinary steroid profiles prior to oocyte pick-up and three weeks after embryo transfer. Setting. University hospital. Sample. Eleven women in the same age range with pregnancy after in vitro fertilization and eleven women who failed to achieve pregnancy. Methods. The standard "long" protocol was used for ovarian stimulation and intracytoplasmic sperm injection for assisted in vitro fertilization. The steroid metabolites in urine samples collected for 24 h were determined by gas chromatography-mass spectrometry. Main outcome measures. Steroid metabolite levels in urine samples determined in the early pregnancy period. Results. The levels of androsterone, etiocholanolone, pregnanediol, tetrahydro-11- dehydrocorticosterone and tetrahydro-corticosterone were significantly higher (p <0.05) in the urine of women with successful pregnancy three weeks after the embryo transfer, while the levels of tetrahydrocortisone, tetrahydrocortisol, allo-tetrahydrocortisol and α-cortolone became higher in the group of patients with unsuccessful pregnancy. Conclusions. The production of androgens, progesterone and corticoid steroid metabolites is altered in the early pregnancy period after in vitro fertilization.

KW - early pregnancy

KW - gas chromatography-mass spectrometry

KW - In vitro fertilization

KW - urinary steroids

UR - http://www.scopus.com/inward/record.url?scp=84860231413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860231413&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0412.2012.01387.x

DO - 10.1111/j.1600-0412.2012.01387.x

M3 - Article

C2 - 22375705

AN - SCOPUS:84860231413

VL - 91

SP - 625

EP - 629

JO - Acta Obstetricia et Gynecologica Scandinavica, Supplement

JF - Acta Obstetricia et Gynecologica Scandinavica, Supplement

SN - 0001-6349

IS - 5

ER -