Urinary orosomucoid: Validation of an automated immune turbidimetric test and its possible clinical use

Péter Kustán, Balázs Szirmay, Zoltán Horváth-Szalai, Andrea Ludány, Ágnes Lakatos, Diána Mühl, Per Hjort Christensen, Attila Miseta, Gábor L. Kovács, Tamás Kőszegi

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4 Citations (Scopus)

Abstract

Introduction: Besides routine serum markers of inflammatory diseases, the diagnostic potential of selected urinary proteins has not been fully exploited yet. Former studies revealed that urinary orosomucoid (u-ORM) might have complementary information in inflammatory disorders. Our aim was to develop and validate a fully automated method for u-ORM measurements and to evaluate its potential clinical impact on systemic inflammatory diseases. Materials and methods: A particle-enhanced immune turbidimetric assay was validated for a Cobas 8000/c502 analyzer to determine u-ORM levels. Spot urine samples from 72 healthy individuals, 28 patients with Crohn’s disease and 30 septic patients were studied. Results: Our assay time was 10 minutes and the detection limit of u-ORM was 0.02 mg/L. The intra- and inter-assay imprecision expressed as CV was less than 5%, and the recovery ranged between 95-103%. Within 10 to 60 years of age, a preliminary reference range for urinary orosomucoid/ creatinine ratio (u-ORM/u-CREAT) was found to be 0.08 (0.01-0.24) mg/mmol [median (2.5-97.5 percentiles)]. Compared to controls, a five-fold increase of u-ORM/u-CREAT values in Crohn’s disease and approximately a 240-fold increase in sepsis were observed. Conclusions: We set up a fast, sensitive and precise turbidimetric approach for automated u-ORM determination. Our highly sensitive assay is ideal for routine u-ORM measurements and might be a potential novel laboratory test in the management of systemic inflammatory processes.

Original languageEnglish
Pages (from-to)421-430
Number of pages10
JournalBiochemia Medica
Volume26
Issue number3
DOIs
Publication statusPublished - Oct 2016

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Keywords

  • Biomarker
  • Inflammation
  • Orosomucoid
  • Particle-enhanced turbidimetry
  • Urine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Kustán, P., Szirmay, B., Horváth-Szalai, Z., Ludány, A., Lakatos, Á., Mühl, D., Christensen, P. H., Miseta, A., Kovács, G. L., & Kőszegi, T. (2016). Urinary orosomucoid: Validation of an automated immune turbidimetric test and its possible clinical use. Biochemia Medica, 26(3), 421-430. https://doi.org/10.11613/BM.2016.044