Uridine release during aminopyridine-induced epilepsy

A. Slézia, K. Kékesi, T. Szikra, A. M. Papp, K. Nagy, M. Szente, Z. Maglóczky, T. Freund, G. Juhász

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Uridine, like adenosine, is released under sustained depolarization and it can inhibit hippocampal neuronal activity, suggesting that uridine may be released during seizures and can be involved in epileptic mechanisms. In an in vivo microdialysis study, we measured the extracellular changes of nucleoside and amino acid levels and recorded cortical EEG during 3-aminopyridine-induced epilepsy. Applying silver impregnation and immunohistochemistry, we examined the degree of hippocampal cell loss. We found that extracellular concentration of uridine, adenosine, inosine, and glutamate increased significantly, while glutamine level decreased during seizures. The release of uridine correlated with seizure activity. Systemic and local uridine application was ineffective. The number of parvalbumin- and calretinin-containing interneurons of dorsal hippocampi decreased. We conclude that uridine is released during epileptic activity, and suggest that as a neuromodulator, uridine may contribute to epilepsy-related neuronal activity changes, but uridine analogues having slower turnover would be needed for further investigation of physiological role of uridine.

Original languageEnglish
Pages (from-to)490-499
Number of pages10
JournalNeurobiology of Disease
Volume16
Issue number3
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Aminopyridines
Uridine
Epilepsy
Seizures
Adenosine
Calbindin 2
Parvalbumins
Inosine
Microdialysis
Interneurons
Glutamine
Nucleosides
Silver
Neurotransmitter Agents
Glutamic Acid
Electroencephalography
Hippocampus
Immunohistochemistry

Keywords

  • 3-aminopyridine
  • 3-AP
  • 4-aminopyridine
  • 4-AP
  • ABC
  • ACSF
  • Adenosine
  • artificial cerebrospinal fluid
  • avidin-biotin peroxidase complex
  • CA1
  • CA2
  • EEG
  • Epilepsy
  • hippocampal Cornu Ammonis 1
  • hippocampal Cornu Ammonis 2
  • Hippocampus
  • Inosine
  • Microdialysis
  • Rat
  • Uridine

ASJC Scopus subject areas

  • Neurology

Cite this

Uridine release during aminopyridine-induced epilepsy. / Slézia, A.; Kékesi, K.; Szikra, T.; Papp, A. M.; Nagy, K.; Szente, M.; Maglóczky, Z.; Freund, T.; Juhász, G.

In: Neurobiology of Disease, Vol. 16, No. 3, 08.2004, p. 490-499.

Research output: Contribution to journalArticle

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