Uricase enzyme types immobilized in poly-N-methylpyrrole for biosensor applications

H. Sántha, R. Dobay, G. Harsányi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The suitability of three different enzymes (from porcine liver, from Candida Utilis, from Bacillus Fastidiosus) have been investigated and significant differences have been found. The results indicate, that beside specific activity also the molecular structure of the enzymes must be taken into account by a biosensor application. A new type of electronically-conducting-polymer based enzymatic uric acid biosensor has been investigated. The layout of the substrate, which consists of 4 electrode surfaces (Working1, Working2, Reference, Counter) was prepared by thick film technology on sintered alumina ceramic sheets. Dodecyl sulfate doped poly-N-methyl-pyrrole layer was used for enzyme immobilization. This polymer film can incorporate the enzyme in one step during the process of the electropolymerization. By the applied bipotentiostatic measurement method the two working electrodes (with and without the enzyme) are identically prepared and polarized and the currents in the two circuits are measured simultaneously. The concentration of the uric acid is calculated from the current difference.

Original languageEnglish
Title of host publication2nd International IEEE Conference on Polymers and Adhesives in Microelectronics and Photonics, POLYTRONIC 2002 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages125-130
Number of pages6
ISBN (Print)078037567X, 9780780375673
DOIs
Publication statusPublished - 2002
Event2nd International IEEE Conference on Polymers and Adhesives in Microelectronics and Photonics, POLYTRONIC 2002 - Zalaegerszeg, Hungary
Duration: Jun 23 2002Jun 26 2002

Other

Other2nd International IEEE Conference on Polymers and Adhesives in Microelectronics and Photonics, POLYTRONIC 2002
CountryHungary
CityZalaegerszeg
Period6/23/026/26/02

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Keywords

  • N-methyl-pyrrole
  • uric acid sensor
  • uricase enzyme

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials

Cite this

Sántha, H., Dobay, R., & Harsányi, G. (2002). Uricase enzyme types immobilized in poly-N-methylpyrrole for biosensor applications. In 2nd International IEEE Conference on Polymers and Adhesives in Microelectronics and Photonics, POLYTRONIC 2002 - Conference Proceedings (pp. 125-130). [1020195] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/POLYTR.2002.1020195