Uptake of isolated plant chromosomes by plant protoplasts

L. Szabados, Gy Hadlaczky, D. Dudits

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticum monococcum) and parsley (Petroselinum hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the metaphase stage. Mitotic protoplasts isolated from these synchronized cell cultures served as a source for isolation of chromosomes. The described isolation and purification method yielded relatively pure chromosome suspension. The uptake of the isolated plant chromosomes into recipient wheat, parsley, and maize protoplasts was induced by polyethylene-glycol treatment. Cytological studies provided evidences for uptake of plant chromosomes into plant protoplasts.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalPlanta
Volume151
Issue number2
DOIs
Publication statusPublished - Feb 1981

Fingerprint

Plant Chromosomes
Petroselinum
Protoplasts
protoplasts
parsley
Triticum
chromosomes
Metaphase
Suspensions
metaphase
Chromosomes
Hydroxyurea
Petroselinum crispum
Colchicine
Triticum monococcum
hydroxyurea
purification methods
wheat
Zea mays
isolation techniques

Keywords

  • Chromosome isolation
  • Petroselinum
  • Polyethylene-glycol
  • Protoplast (uptake of chromosomes)
  • Triticum
  • Zea

ASJC Scopus subject areas

  • Plant Science

Cite this

Uptake of isolated plant chromosomes by plant protoplasts. / Szabados, L.; Hadlaczky, Gy; Dudits, D.

In: Planta, Vol. 151, No. 2, 02.1981, p. 141-145.

Research output: Contribution to journalArticle

Szabados, L. ; Hadlaczky, Gy ; Dudits, D. / Uptake of isolated plant chromosomes by plant protoplasts. In: Planta. 1981 ; Vol. 151, No. 2. pp. 141-145.
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