Uptake of arachidonic acid, arachidic acid, oleic acid and their incorporation into phospholipids and triacylglycerols of isolated murine hepatocytes. Effect of thrombin-antithrombin III complex

J. Mandl, B. Tanács, Z. Spolarics, T. Garzó, I. Mucha, Fw Antoni, R. Machovich, I. Horvath

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Uptake and metabolism of arachidonic acid, arachidic acid and oleic acid were investigated in isolated hepatocytes prepared from mouse liver with the collagenase perfusion method. The rate of uptake of arachidonic acid was time- and concentration- dependent. 94-98% of the arachidonic acid was incorporated into the phospholipid and triacylglycers1 fractions following a 60 min incubation period at 37°C. In the presence of thrombin-anti-thrombin III complex a change in the distribution of arachidonic acid incorporated into lipid fractions was found, i.e. increased incorporation into phosphatidyl-serine and phosphatidylethanolamine, whereas the uptake was not altered. There was no change in the uptake and incorporation of arachidic acid and oleic acid.

Original languageEnglish
Pages (from-to)407-414
Number of pages8
JournalThrombosis research
Volume35
Issue number4
DOIs
Publication statusPublished - Aug 15 1984

Keywords

  • arachidonic acid
  • hepatocytes
  • thrombin-antithrombin III complex

ASJC Scopus subject areas

  • Hematology

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