Up-regulation of vasopressin mRNA in paraventricular hypophysiotrophic neurons after acute immobilization stress

Viktor Baitanusz, Jean Michel Aubry, Daniela Jezova, Judit Baffi, J. Kiss

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Cellular levels of vasopressin (VP) and corticotropin-releasing factor (CRF) mRNAs were determined in the rat hypothalamic paraventricular nucleus after acute immobilization stress. Messenger RNA levels were measured by in situ hybridization histochemistry using, 35S-labeled synthetic DNA oligonucleotide probes and quantitative autoradiography. Analysis of histograms related to density values revealed that two hours after immobilization the average cellular VP and CRF mRNA levels were significantly increased (145 ± 30.3 and 68 ± 21.3%, respectively, above control values). Moreover, the number of VP-expressing parvicellular neurons was doubled in stressed rats relative to controls. These results indicate that single acute immobilization stress is sufficient to up-regulate VP as well as CRF mRNA production in the hypothalamic CRF-neurosecretory system.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalNeuroendocrinology
Volume58
Issue number6
DOIs
Publication statusPublished - 1993

Fingerprint

Corticotropin-Releasing Hormone
Vasopressins
Immobilization
Up-Regulation
Neurons
Messenger RNA
Pituitary Hormone-Releasing Hormones
Neurosecretory Systems
Oligonucleotide Probes
Paraventricular Hypothalamic Nucleus
DNA Probes
Autoradiography
In Situ Hybridization

Keywords

  • Corticotropin-releasing factor
  • Hypothalamic paraventricular nucleus
  • In situ hybridization

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Up-regulation of vasopressin mRNA in paraventricular hypophysiotrophic neurons after acute immobilization stress. / Baitanusz, Viktor; Aubry, Jean Michel; Jezova, Daniela; Baffi, Judit; Kiss, J.

In: Neuroendocrinology, Vol. 58, No. 6, 1993, p. 625-629.

Research output: Contribution to journalArticle

Baitanusz, Viktor ; Aubry, Jean Michel ; Jezova, Daniela ; Baffi, Judit ; Kiss, J. / Up-regulation of vasopressin mRNA in paraventricular hypophysiotrophic neurons after acute immobilization stress. In: Neuroendocrinology. 1993 ; Vol. 58, No. 6. pp. 625-629.
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