Up-regulation of P2X7 receptor-immunoreactivity by in vitro ischemia on the plasma membrane of cultured rat cortical neurons

Doreen Milius, B. Sperlágh, Peter Illes

Research output: Contribution to journalArticle

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Abstract

Mixed neuronal/astrocytic cortical cell cultures of the rat were incubated for 2 or 12 h under normoxic or ischemic conditions. Subsequent flow cytometric analysis with an anti-P2X7 receptor antibody directed against an extracellular epitope indicated the up-regulation of these receptors at the plasma membrane by 12 h of ischemia. Labelling of MAP-2 immunopositive neurons by an anti-P2X7 antibody directed against a C-terminal epitope, documented the selectivity of the ischemia-induced increase in receptor-density for the neuronal population. By contrast, staining of GFAP immunopositive astrocytes by the same anti-P2X7 antibody excluded any effect of ischemia on the astrocytic density of P2X7 receptors. The ischemic up-regulation of neuronal P2X7 receptors is in perfect agreement with the previously reported facilitation of transmitter release from the GABAergic non-pyramidal cell type in such cultures [K. Wirkner, A. Köfalvi, W. Fischer, A. Günther, H. Franke, H. Gröger-Arndt, W. Nörenberg, E. Madarasz, E.S. Vizi, D. Schneider, B. Sperlagh, P. Illes, Supersensitivity of P2X7 receptors in cerebrocortical cell cultures after in vitro ischemia, J. Neurochem. 95 (2005) 1421-1437].

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalNeuroscience Letters
Volume446
Issue number1
DOIs
Publication statusPublished - Nov 28 2008

Fingerprint

Purinergic P2X7 Receptors
Up-Regulation
Ischemia
Cell Membrane
Neurons
Epitopes
Anti-Idiotypic Antibodies
Cell Culture Techniques
Population Density
Astrocytes
Staining and Labeling
In Vitro Techniques
Antibodies

Keywords

  • Flow cytometry
  • In vitro ischemia
  • Neuronal-astrocytic cultures
  • P2X receptor-immunoreactivity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Up-regulation of P2X7 receptor-immunoreactivity by in vitro ischemia on the plasma membrane of cultured rat cortical neurons. / Milius, Doreen; Sperlágh, B.; Illes, Peter.

In: Neuroscience Letters, Vol. 446, No. 1, 28.11.2008, p. 45-50.

Research output: Contribution to journalArticle

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