Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets

Antal Nógrádi, F. Domoki, Rózsa Dégi, Stefánia Borda, M. Pákáski, András Szabó, F. Bari

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The resuscitation of asphyxiated babies is associated with changes in cerebral protein synthesis that can influence the neurological outcome. Insufficient gas exchange results in rapid shifts in extracellular and intracellular pH. Carbonic anhydrase (CA) plays an important role in buffering acute changes in pH in the brain. We investigated whether asphyxia/re-ventilation influences the expression of cerebral CA isoforms (CA-II, CA-III and CA-IV) in anaesthetized newborn pigs. The cerebral cortex, hippocampus, cerebellum and retina were sampled, and prepared for either CA immunohistochemistry or CA immunoblotting from piglets subjected to asphyxia (10 min) followed by 2-4 h of re-ventilation, and also from normoxic controls. The CA immunoreactivity (IR) of all the isoforms studied was weak in the controls, apart from staining of a few oligodendrocytes in the subcortical white matter, some astrocytes in the superficial layer of the cerebral cortex, the cerebellar Purkinje cells and the retinal Müller cells that possessed moderate CA-II IR. However, asphyxia induced a marked increase in the CA IR of all isoforms in all the cerebral regions investigated and the retina after 4 h of survival. The pyramidal cells of the frontal cortex and hippocampus displayed the most conspicuous increase in CA IR. Immunoblotting confirmed increased levels of all the CA isoenzymes. We conclude that raised CA levels after asphyxia may contribute to the compensatory mechanisms that protect against the pathological changes in the neonatal CNS.

Original languageEnglish
Pages (from-to)843-850
Number of pages8
JournalJournal of Neurochemistry
Volume85
Issue number4
Publication statusPublished - May 2003

Fingerprint

Carbonic Anhydrases
Up-Regulation
Asphyxia
Carbonic Anhydrase II
Protein Isoforms
Immunoblotting
Cerebral Cortex
Ventilation
Retina
Hippocampus
Carbonic Anhydrase IV
Carbonic Anhydrase III
Resuscitation
Pyramidal Cells
Purkinje Cells
Oligodendroglia
Frontal Lobe
Astrocytes
Cerebellum
Isoenzymes

Keywords

  • Immunohistochemistry
  • Perinatal asphyxia
  • Preconditioning
  • Western blot

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets. / Nógrádi, Antal; Domoki, F.; Dégi, Rózsa; Borda, Stefánia; Pákáski, M.; Szabó, András; Bari, F.

In: Journal of Neurochemistry, Vol. 85, No. 4, 05.2003, p. 843-850.

Research output: Contribution to journalArticle

Nógrádi, Antal ; Domoki, F. ; Dégi, Rózsa ; Borda, Stefánia ; Pákáski, M. ; Szabó, András ; Bari, F. / Up-regulation of cerebral carbonic anhydrase by anoxic stress in piglets. In: Journal of Neurochemistry. 2003 ; Vol. 85, No. 4. pp. 843-850.
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