Up-regulation of antioxidants in tobacco by low concentrations of H 2O 2 suppresses necrotic disease symptoms

Yaser Mohamed Hafez, Renáta Bacsó, Z. Király, András Künstler, Lóránt Király

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Pretreatment of tobacco leaves with low concentrations (5 to 10 mM) of H 2O 2 suppressed hypersensitive-type necrosis associated with resistance to Tobacco mosaic virus (TMV) or Pseudomonas syringae pv. phaseolicola. The same pretreatment resulted in suppression of normosensitive necrosis associated with susceptibility to Botrytis cinerea. This type of H 2O 2-mediated, induced disease symptom resistance correlated with enhanced host antioxidant capacity, i.e., elevated enzymatic activities of catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (POX) after viral and bacterial infections. Induction of genes that encode the antioxidants superoxide dismutase (SOD), CAT, and APX was also enhanced early after TMV infection. Artificial application of SOD and CAT suppressed necroses caused by viral, bacterial, or fungal pathogens similarly as H 2O 2 pretreatment, implying that H 2O 2-mediated symptom resistance operates through enhancement of plant antioxidant capacity. Pathogen multiplication was not significantly affected in H 2O 2- pretreated plants. Salicylic acid (SA), a central component of plant defense, does not seem to function in this type of H 2O 2-mediated symptom resistance, indicated by unchanged levels of free and bound SA and a lack of early up-regulation of an SA glucosyltransferase gene in TMV-infected H 2O 2-pretreated tobacco. Taken together, H 2O 2-mediated, induced resistance to necrotic symptoms in tobacco seems to depend on enhanced antioxidant capacity.

Original languageEnglish
Pages (from-to)848-856
Number of pages9
JournalPhytopathology
Volume102
Issue number9
DOIs
Publication statusPublished - Sep 2012

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signs and symptoms (plants)
Tobacco mosaic virus
tobacco
salicylic acid
antioxidants
catalase
necrosis
pretreatment
ascorbate peroxidase
superoxide dismutase
Pseudomonas syringae pv. phaseolicola
glucosyltransferases
pathogens
gene induction
induced resistance
Botrytis cinerea
bacterial infections
peroxidase
infection
leaves

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

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Up-regulation of antioxidants in tobacco by low concentrations of H 2O 2 suppresses necrotic disease symptoms. / Hafez, Yaser Mohamed; Bacsó, Renáta; Király, Z.; Künstler, András; Király, Lóránt.

In: Phytopathology, Vol. 102, No. 9, 09.2012, p. 848-856.

Research output: Contribution to journalArticle

Hafez, Yaser Mohamed ; Bacsó, Renáta ; Király, Z. ; Künstler, András ; Király, Lóránt. / Up-regulation of antioxidants in tobacco by low concentrations of H 2O 2 suppresses necrotic disease symptoms. In: Phytopathology. 2012 ; Vol. 102, No. 9. pp. 848-856.
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