Up-regulated transcripts in a compatible powdery mildew-grapevine interaction

C. Fekete, Raymond W M Fung, Zoltán Szabó, Wenping Qiu, Le Chang, Daniel P. Schachtman, László G. Kovács

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Powdery mildews (Erysiphales) are obligate biotrophic pathogens that invade susceptible plant cells without triggering cell death. This suggests a highly adept mechanism of parasitism which enables powdery mildews to avoid detection or evade defenses by their host. To better understand this plant-pathogen interaction, we employed suppression subtractive hybridization (SSH), differential hybridization and quantitative real-time (qRT) PCR for the identification of grapevine (Vitis vinifera L.) genes that were specifically up-regulated in response to the grape powdery mildew Erysiphe necator Schwein. We identified 25 grapevine transcripts that increased in abundance upon infection in leaves of the susceptible host V. vinifera Cabernet Sauvignon. Despite the compatible interaction between the pathogen and plant, several of the E. necator-induced transcripts represented typical defense response genes. Among the transcripts identified were those that encoded a leucine-rich repeat serine/threonine kinase-like receptor, an MYB transcription factor, and two ubiquitination-associated proteins, indicating the stimulation of intracellular signal transduction and regulatory functions. A number of genes characteristic of senescence processes, including metallothioneins, a deoxyribonuclease, an aspartyl protease and a subtilase-like serine protease, also were identified. These transcripts expanded the list of previously identified E. necator-responsive grapevine genes and facilitated a more comprehensive view of the molecular events that underlie this economically important plant-pathogen interaction.

Original languageEnglish
Pages (from-to)732-738
Number of pages7
JournalPlant Physiology and Biochemistry
Volume47
Issue number8
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Necator
Uncinula necator
powdery mildew
Vitis
mildews
Vitis vinifera
plant pathogens
Genes
genes
Erysiphales
Aspartic Acid Proteases
aspartic proteinases
deoxyribonucleases
suppression subtractive hybridization
Deoxyribonucleases
pathogens
Metallothionein
Protein-Serine-Threonine Kinases
Ubiquitination
Plant Cells

Keywords

  • Defense
  • Grapevine
  • Powdery mildew
  • Susceptibility
  • Transcription

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Fekete, C., Fung, R. W. M., Szabó, Z., Qiu, W., Chang, L., Schachtman, D. P., & Kovács, L. G. (2009). Up-regulated transcripts in a compatible powdery mildew-grapevine interaction. Plant Physiology and Biochemistry, 47(8), 732-738. https://doi.org/10.1016/j.plaphy.2009.03.006

Up-regulated transcripts in a compatible powdery mildew-grapevine interaction. / Fekete, C.; Fung, Raymond W M; Szabó, Zoltán; Qiu, Wenping; Chang, Le; Schachtman, Daniel P.; Kovács, László G.

In: Plant Physiology and Biochemistry, Vol. 47, No. 8, 08.2009, p. 732-738.

Research output: Contribution to journalArticle

Fekete, C, Fung, RWM, Szabó, Z, Qiu, W, Chang, L, Schachtman, DP & Kovács, LG 2009, 'Up-regulated transcripts in a compatible powdery mildew-grapevine interaction', Plant Physiology and Biochemistry, vol. 47, no. 8, pp. 732-738. https://doi.org/10.1016/j.plaphy.2009.03.006
Fekete, C. ; Fung, Raymond W M ; Szabó, Zoltán ; Qiu, Wenping ; Chang, Le ; Schachtman, Daniel P. ; Kovács, László G. / Up-regulated transcripts in a compatible powdery mildew-grapevine interaction. In: Plant Physiology and Biochemistry. 2009 ; Vol. 47, No. 8. pp. 732-738.
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