Unsaturation of fatty acids in membrane lipids enhances tolerance of the cyanobacterium Synechocystis PCC6803 to low-temperature photoinhibition

Z. Gombos, Hajime Wada, Norio Murata

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Abstract

Effect of the unsaturation of fatty acids in the glycerolipids of thylakoid membranes on low-temperature photoinhibition of photosynthesis was studied by mutation and transformation of the cyanobacterium Synechocystis PCC6803. When grown at 34°C, the wild type contained mono-, di-, and triunsaturated lipids; a mutant, designated Fad6, contained mono- and diunsaturated lipids; and a transformant of Fad6, with a disrupted gene for desaturation and designated Fad6/desA::Kmr, contained only monounsaturated lipids. Fad6/desA::Kmr was the most susceptible among these strains to low-temperature photoinhibition of photosynthesis, whereas Fad6 and the wild type were apparently indistinguishable in terms of sensitivity to photoinhibition. This result suggests that the presence of diunsaturated fatty acids is important in protecting against low-temperature photoinhibition. The photoinhibition at room temperature, although much less significant than that at low temperature, was also affected by the unsaturation of fatty acids. By contrast, the photosynthetic transport of electrons, measured at various temperatures, was not affected by changes in extent of fatty acid unsaturation.

Original languageEnglish
Pages (from-to)9959-9963
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number20
Publication statusPublished - Oct 15 1992

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Synechocystis
Cyanobacteria
Membrane Lipids
Fatty Acids
Temperature
Photosynthesis
Lipids
Thylakoids
Electron Transport
Mutation
Genes

Keywords

  • Chilling sensitivity
  • Photosynthesis
  • Polyunsaturated fatty acid

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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abstract = "Effect of the unsaturation of fatty acids in the glycerolipids of thylakoid membranes on low-temperature photoinhibition of photosynthesis was studied by mutation and transformation of the cyanobacterium Synechocystis PCC6803. When grown at 34°C, the wild type contained mono-, di-, and triunsaturated lipids; a mutant, designated Fad6, contained mono- and diunsaturated lipids; and a transformant of Fad6, with a disrupted gene for desaturation and designated Fad6/desA::Kmr, contained only monounsaturated lipids. Fad6/desA::Kmr was the most susceptible among these strains to low-temperature photoinhibition of photosynthesis, whereas Fad6 and the wild type were apparently indistinguishable in terms of sensitivity to photoinhibition. This result suggests that the presence of diunsaturated fatty acids is important in protecting against low-temperature photoinhibition. The photoinhibition at room temperature, although much less significant than that at low temperature, was also affected by the unsaturation of fatty acids. By contrast, the photosynthetic transport of electrons, measured at various temperatures, was not affected by changes in extent of fatty acid unsaturation.",
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AU - Wada, Hajime

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N2 - Effect of the unsaturation of fatty acids in the glycerolipids of thylakoid membranes on low-temperature photoinhibition of photosynthesis was studied by mutation and transformation of the cyanobacterium Synechocystis PCC6803. When grown at 34°C, the wild type contained mono-, di-, and triunsaturated lipids; a mutant, designated Fad6, contained mono- and diunsaturated lipids; and a transformant of Fad6, with a disrupted gene for desaturation and designated Fad6/desA::Kmr, contained only monounsaturated lipids. Fad6/desA::Kmr was the most susceptible among these strains to low-temperature photoinhibition of photosynthesis, whereas Fad6 and the wild type were apparently indistinguishable in terms of sensitivity to photoinhibition. This result suggests that the presence of diunsaturated fatty acids is important in protecting against low-temperature photoinhibition. The photoinhibition at room temperature, although much less significant than that at low temperature, was also affected by the unsaturation of fatty acids. By contrast, the photosynthetic transport of electrons, measured at various temperatures, was not affected by changes in extent of fatty acid unsaturation.

AB - Effect of the unsaturation of fatty acids in the glycerolipids of thylakoid membranes on low-temperature photoinhibition of photosynthesis was studied by mutation and transformation of the cyanobacterium Synechocystis PCC6803. When grown at 34°C, the wild type contained mono-, di-, and triunsaturated lipids; a mutant, designated Fad6, contained mono- and diunsaturated lipids; and a transformant of Fad6, with a disrupted gene for desaturation and designated Fad6/desA::Kmr, contained only monounsaturated lipids. Fad6/desA::Kmr was the most susceptible among these strains to low-temperature photoinhibition of photosynthesis, whereas Fad6 and the wild type were apparently indistinguishable in terms of sensitivity to photoinhibition. This result suggests that the presence of diunsaturated fatty acids is important in protecting against low-temperature photoinhibition. The photoinhibition at room temperature, although much less significant than that at low temperature, was also affected by the unsaturation of fatty acids. By contrast, the photosynthetic transport of electrons, measured at various temperatures, was not affected by changes in extent of fatty acid unsaturation.

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