Unraveling the stability of plasma proteins upon interaction of synthesized androstenedione and its derivatives-a biophysical and computational approach

Aparna Nerusu, P. Srinivasa Reddy, Dhevalapally B. Ramachary, S. Rajagopal

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

4-Androstene-3-17-dione (4A), also known as androstenedione, is the key intermediate of steroid metabolism. 5β-Androstane-3-17-dione (5A) and (+)-6-methyl-5β-androstane- 3-17-dione (6M) are the steroid derivatives of androstenedione. The interactions of androstenedione and its derivatives with plasma proteins are important in understanding the distribution and bioavailability of these molecules. In our present study, we have studied the binding affinity of androstenedione and its derivatives with plasma proteins such as human serum albumin (HSA) and α1-acid glycoprotein (AGP). Our results showed that the 4A, 5A, and 6M steroid molecules can form stable complexes with HSA and AGP. The affinity of the studied steroid molecules with HSA is high compared to that with AGP, and the binding constants obtained for 4A, 5A, and 6M with HSA are 5.3 ± 2 × 104, 5.3 ± 1 × 104, and 9.5 ± 0.2 × 104 M-1, respectively. Further, binding sites of these steroid molecules in HSA are identified using molecular displacement and docking studies: it is found that 4A and 5A bind to domain III while 6M binds to domain II of HSA. Furthermore, the circular dichroism data revealed that there is a partial unfolding of the protein while interacting with androstenedione and its derivatives. Also, molecular dynamics simulations were carried out for HSA-androstenedione and its derivative complexes to understand their stability; hence, these results yielded that HSA-androstenedione and its derivative complexes were stabilized after 15 ns and maintained their stable structures.

Original languageEnglish
Pages (from-to)6514-6524
Number of pages11
JournalACS Omega
Volume2
Issue number10
DOIs
Publication statusPublished - Jan 1 2017

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Androstenedione
Beam plasma interactions
Serum Albumin
Blood Proteins
Derivatives
Proteins
Plasmas
Glycoproteins
Steroids
Molecules
Acids
Androstenes
Dichroism
Binding sites
Metabolism
Molecular dynamics
Binding Sites
Computer simulation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

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Unraveling the stability of plasma proteins upon interaction of synthesized androstenedione and its derivatives-a biophysical and computational approach. / Nerusu, Aparna; Reddy, P. Srinivasa; Ramachary, Dhevalapally B.; Rajagopal, S.

In: ACS Omega, Vol. 2, No. 10, 01.01.2017, p. 6514-6524.

Research output: Contribution to journalArticle

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