Unoprostone isopropyl pretreatment decreases endothelin-1 release and the intra-ocular pressure spike induced by laser trabeculoplasty in the rabbit

G. Holló, Zsuzsanna Visontai, P. Lakatos, P. Vargha

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4 Citations (Scopus)

Abstract

Purpose: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. In this study we investigated whether pretreatment with topical unoprostone isopropyl, a functional antagonist of ET-1, protects against the pressure spike due to ALT in the rabbit model. Methods: IOP of both eyes of 17 pigmented rabbits was measured with a TonoPen XL tonometer under general anaesthesia (baseline measurement). Then the right eyes were treated with topical unoprostone isopropyl 0.12% BID for 6 days, and the left eyes similarly received balanced salt solution (BSS) twice daily. After the last morning instillation, IOP for both eyes was measured under general anaesthesia after 2 h in group 1 and after 3 h in group 2 animals. Then, for both groups, ALT was performed on both eyes. Thirty minutes after laser treatment (and still under general anaesthesia), IOP was measured again. Then the aqueous humour was aspirated for measurement of ET-1 concentration. Results: Baseline individual IOP differences (left eye minus right eye) were not significant in both groups: mean ± SD 0.25 ± 1.16 and -0.33 ± 0.71 mm Hg, respectively (paired t test, p > 0.05 for both groups). On day 6, 2 h (group 1) and 3 h (group 2) after the last instillation but before laser trabeculoplasty, the difference (BSS-pretreated left eye minus unoprostone-pretreated right eye) increased to 2.13 ± 2.10 mm Hg (p =0.024) and 1.33 ± 0.71 mm Hg (p =0.0005), respectively. Thirty minutes after ALT, the IOP difference was 2.63 ± 2.67 mm Hg in group 1 (p =0.027), but no difference was seen in group 2 (0.67 ± 1.94 mm Hg, p =0.332). In group 1, postoperative aqueous humour ET-1 concentration for the unoprostone-pretreated right eye was significantly lower than that for the BSS-pretreated left eye (paired t test, p =0.01); but in group 2 no inter-ocular difference in ET-1 concentration was found (p =0.976). Conclusion: Topical unoprostone pretreatment decreases laser-induced ET-1 release and diminishes the IOP spike caused by ALT in vivo within 2 h after instillation, when the concentration of unoprostone in the aqueous humour is high.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalOphthalmologica
Volume217
Issue number3
DOIs
Publication statusPublished - 2003

Fingerprint

Trabeculectomy
Endothelin-1
Lasers
Rabbits
Pressure
Argon
Aqueous Humor
isopropyl unoprostone
General Anesthesia
Salts

Keywords

  • Argon laser trabeculoplasty
  • Endothelin antagonists
  • Glaucoma
  • Intra-ocular pressure
  • Rescula®
  • Unoprostone isopropyl

ASJC Scopus subject areas

  • Ophthalmology

Cite this

@article{280178b996c64909bd6843bde46be45c,
title = "Unoprostone isopropyl pretreatment decreases endothelin-1 release and the intra-ocular pressure spike induced by laser trabeculoplasty in the rabbit",
abstract = "Purpose: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. In this study we investigated whether pretreatment with topical unoprostone isopropyl, a functional antagonist of ET-1, protects against the pressure spike due to ALT in the rabbit model. Methods: IOP of both eyes of 17 pigmented rabbits was measured with a TonoPen XL tonometer under general anaesthesia (baseline measurement). Then the right eyes were treated with topical unoprostone isopropyl 0.12{\%} BID for 6 days, and the left eyes similarly received balanced salt solution (BSS) twice daily. After the last morning instillation, IOP for both eyes was measured under general anaesthesia after 2 h in group 1 and after 3 h in group 2 animals. Then, for both groups, ALT was performed on both eyes. Thirty minutes after laser treatment (and still under general anaesthesia), IOP was measured again. Then the aqueous humour was aspirated for measurement of ET-1 concentration. Results: Baseline individual IOP differences (left eye minus right eye) were not significant in both groups: mean ± SD 0.25 ± 1.16 and -0.33 ± 0.71 mm Hg, respectively (paired t test, p > 0.05 for both groups). On day 6, 2 h (group 1) and 3 h (group 2) after the last instillation but before laser trabeculoplasty, the difference (BSS-pretreated left eye minus unoprostone-pretreated right eye) increased to 2.13 ± 2.10 mm Hg (p =0.024) and 1.33 ± 0.71 mm Hg (p =0.0005), respectively. Thirty minutes after ALT, the IOP difference was 2.63 ± 2.67 mm Hg in group 1 (p =0.027), but no difference was seen in group 2 (0.67 ± 1.94 mm Hg, p =0.332). In group 1, postoperative aqueous humour ET-1 concentration for the unoprostone-pretreated right eye was significantly lower than that for the BSS-pretreated left eye (paired t test, p =0.01); but in group 2 no inter-ocular difference in ET-1 concentration was found (p =0.976). Conclusion: Topical unoprostone pretreatment decreases laser-induced ET-1 release and diminishes the IOP spike caused by ALT in vivo within 2 h after instillation, when the concentration of unoprostone in the aqueous humour is high.",
keywords = "Argon laser trabeculoplasty, Endothelin antagonists, Glaucoma, Intra-ocular pressure, Rescula{\circledR}, Unoprostone isopropyl",
author = "G. Holl{\'o} and Zsuzsanna Visontai and P. Lakatos and P. Vargha",
year = "2003",
doi = "10.1159/000068972",
language = "English",
volume = "217",
pages = "231--236",
journal = "Ophthalmologica",
issn = "0030-3755",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Unoprostone isopropyl pretreatment decreases endothelin-1 release and the intra-ocular pressure spike induced by laser trabeculoplasty in the rabbit

AU - Holló, G.

AU - Visontai, Zsuzsanna

AU - Lakatos, P.

AU - Vargha, P.

PY - 2003

Y1 - 2003

N2 - Purpose: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. In this study we investigated whether pretreatment with topical unoprostone isopropyl, a functional antagonist of ET-1, protects against the pressure spike due to ALT in the rabbit model. Methods: IOP of both eyes of 17 pigmented rabbits was measured with a TonoPen XL tonometer under general anaesthesia (baseline measurement). Then the right eyes were treated with topical unoprostone isopropyl 0.12% BID for 6 days, and the left eyes similarly received balanced salt solution (BSS) twice daily. After the last morning instillation, IOP for both eyes was measured under general anaesthesia after 2 h in group 1 and after 3 h in group 2 animals. Then, for both groups, ALT was performed on both eyes. Thirty minutes after laser treatment (and still under general anaesthesia), IOP was measured again. Then the aqueous humour was aspirated for measurement of ET-1 concentration. Results: Baseline individual IOP differences (left eye minus right eye) were not significant in both groups: mean ± SD 0.25 ± 1.16 and -0.33 ± 0.71 mm Hg, respectively (paired t test, p > 0.05 for both groups). On day 6, 2 h (group 1) and 3 h (group 2) after the last instillation but before laser trabeculoplasty, the difference (BSS-pretreated left eye minus unoprostone-pretreated right eye) increased to 2.13 ± 2.10 mm Hg (p =0.024) and 1.33 ± 0.71 mm Hg (p =0.0005), respectively. Thirty minutes after ALT, the IOP difference was 2.63 ± 2.67 mm Hg in group 1 (p =0.027), but no difference was seen in group 2 (0.67 ± 1.94 mm Hg, p =0.332). In group 1, postoperative aqueous humour ET-1 concentration for the unoprostone-pretreated right eye was significantly lower than that for the BSS-pretreated left eye (paired t test, p =0.01); but in group 2 no inter-ocular difference in ET-1 concentration was found (p =0.976). Conclusion: Topical unoprostone pretreatment decreases laser-induced ET-1 release and diminishes the IOP spike caused by ALT in vivo within 2 h after instillation, when the concentration of unoprostone in the aqueous humour is high.

AB - Purpose: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. In this study we investigated whether pretreatment with topical unoprostone isopropyl, a functional antagonist of ET-1, protects against the pressure spike due to ALT in the rabbit model. Methods: IOP of both eyes of 17 pigmented rabbits was measured with a TonoPen XL tonometer under general anaesthesia (baseline measurement). Then the right eyes were treated with topical unoprostone isopropyl 0.12% BID for 6 days, and the left eyes similarly received balanced salt solution (BSS) twice daily. After the last morning instillation, IOP for both eyes was measured under general anaesthesia after 2 h in group 1 and after 3 h in group 2 animals. Then, for both groups, ALT was performed on both eyes. Thirty minutes after laser treatment (and still under general anaesthesia), IOP was measured again. Then the aqueous humour was aspirated for measurement of ET-1 concentration. Results: Baseline individual IOP differences (left eye minus right eye) were not significant in both groups: mean ± SD 0.25 ± 1.16 and -0.33 ± 0.71 mm Hg, respectively (paired t test, p > 0.05 for both groups). On day 6, 2 h (group 1) and 3 h (group 2) after the last instillation but before laser trabeculoplasty, the difference (BSS-pretreated left eye minus unoprostone-pretreated right eye) increased to 2.13 ± 2.10 mm Hg (p =0.024) and 1.33 ± 0.71 mm Hg (p =0.0005), respectively. Thirty minutes after ALT, the IOP difference was 2.63 ± 2.67 mm Hg in group 1 (p =0.027), but no difference was seen in group 2 (0.67 ± 1.94 mm Hg, p =0.332). In group 1, postoperative aqueous humour ET-1 concentration for the unoprostone-pretreated right eye was significantly lower than that for the BSS-pretreated left eye (paired t test, p =0.01); but in group 2 no inter-ocular difference in ET-1 concentration was found (p =0.976). Conclusion: Topical unoprostone pretreatment decreases laser-induced ET-1 release and diminishes the IOP spike caused by ALT in vivo within 2 h after instillation, when the concentration of unoprostone in the aqueous humour is high.

KW - Argon laser trabeculoplasty

KW - Endothelin antagonists

KW - Glaucoma

KW - Intra-ocular pressure

KW - Rescula®

KW - Unoprostone isopropyl

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U2 - 10.1159/000068972

DO - 10.1159/000068972

M3 - Article

VL - 217

SP - 231

EP - 236

JO - Ophthalmologica

JF - Ophthalmologica

SN - 0030-3755

IS - 3

ER -