Unexpected retention behavior of baicalin: Hydrophilic interaction like properties of a reversed-phase column

Balázs Magda, Zoltán Márta, Tímea Imre, Bernadett Kalapos-Kovács, Imre Klebovich, Jeno Fekete, Pál T. Szabó

Research output: Contribution to journalArticle

4 Citations (Scopus)


The original aim of this study was to develop a method for the determination of baicalin from membrane vesicles. The unconventional chromatographic separation ("inverse gradient elution" on a reversed phase column) was due to a lucky chance, which is detailed and discussed in this study. The validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is proved to be sensitive, rapid and selective. Chromatographic separation was performed on a Zorbax SB-C8 column (250. mm. ×. 4.6. mm, i.d.; 5. μm) with 0.1% formic acid in water and methanol by linear gradient elution. Quantification of baicalin was determined by multiple reaction monitoring (MRM) mode using electrospray ionization (ESI). The calibration curve was linear (r= 0.9987) over the concentration range from 1 to 1000. nM. The coefficient of variation and relative error of baicalin for intra- and inter-assay at three quality control (QC) levels were 2.0-10.2% and -6.1 to 6.7%, respectively. The lower limit of quantification (LLOQ) for baicalin was 1. nM (0.446. ng/ml), without preconcentration of the sample. This method was subsequently applied to vesicular transport assays of baicalin in membrane vesicles successfully. The developed method can open up new area of research in the chromatographic separation of flavonoids and their glucuronides.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Publication statusPublished - Jul 1 2015


  • Baicalin
  • Hydrophilic interaction chromatography
  • LC-MS/MS
  • Reversed-phase chromatography
  • Vesicular transport assay

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Unexpected retention behavior of baicalin: Hydrophilic interaction like properties of a reversed-phase column'. Together they form a unique fingerprint.

  • Cite this