Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

Jobin Jose Kattoor, Sharad Saurabh, Yashpal Singh Malik, Shubhankar Sircar, Kuldeep Dhama, Souvik Ghosh, K. Bányai, Nobumichi Kobayashi, Raj Kumar Singh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

BACKGROUND: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog.

OBJECTIVE: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes.

METHODS: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed.

RESULTS: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population.

CONCLUSION: The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

Original languageEnglish
Pages (from-to)252-261
Number of pages10
JournalThe Veterinary quarterly
Volume37
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

Rotavirus C
Rotavirus
Swine
swine
Genes
genes
Population
Genotype
Mink
incidence
Ferrets
genotype
mink
ferrets
Incidence
etiological agents
Zoonoses
Capsid Proteins
polyacrylamide
coat proteins

Keywords

  • human origin
  • I2 genotype
  • Porcine
  • rotavirus C
  • swine
  • VP6 gene
  • zoonosis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Kattoor, J. J., Saurabh, S., Malik, Y. S., Sircar, S., Dhama, K., Ghosh, S., ... Singh, R. K. (2017). Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. The Veterinary quarterly, 37(1), 252-261. https://doi.org/10.1080/01652176.2017.1346849

Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. / Kattoor, Jobin Jose; Saurabh, Sharad; Malik, Yashpal Singh; Sircar, Shubhankar; Dhama, Kuldeep; Ghosh, Souvik; Bányai, K.; Kobayashi, Nobumichi; Singh, Raj Kumar.

In: The Veterinary quarterly, Vol. 37, No. 1, 01.12.2017, p. 252-261.

Research output: Contribution to journalArticle

Kattoor, JJ, Saurabh, S, Malik, YS, Sircar, S, Dhama, K, Ghosh, S, Bányai, K, Kobayashi, N & Singh, RK 2017, 'Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene', The Veterinary quarterly, vol. 37, no. 1, pp. 252-261. https://doi.org/10.1080/01652176.2017.1346849
Kattoor, Jobin Jose ; Saurabh, Sharad ; Malik, Yashpal Singh ; Sircar, Shubhankar ; Dhama, Kuldeep ; Ghosh, Souvik ; Bányai, K. ; Kobayashi, Nobumichi ; Singh, Raj Kumar. / Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene. In: The Veterinary quarterly. 2017 ; Vol. 37, No. 1. pp. 252-261.
@article{543612e5dce84e8abe129e75ddc42587,
title = "Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene",
abstract = "BACKGROUND: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog.OBJECTIVE: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes.METHODS: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed.RESULTS: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0{\%} (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93{\%} homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population.CONCLUSION: The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.",
keywords = "human origin, I2 genotype, Porcine, rotavirus C, swine, VP6 gene, zoonosis",
author = "Kattoor, {Jobin Jose} and Sharad Saurabh and Malik, {Yashpal Singh} and Shubhankar Sircar and Kuldeep Dhama and Souvik Ghosh and K. B{\'a}nyai and Nobumichi Kobayashi and Singh, {Raj Kumar}",
year = "2017",
month = "12",
day = "1",
doi = "10.1080/01652176.2017.1346849",
language = "English",
volume = "37",
pages = "252--261",
journal = "Veterinary Quarterly",
issn = "0165-2176",
publisher = "Koninklijke Nederlandse Maatschappij voor Diergeneeskunde",
number = "1",

}

TY - JOUR

T1 - Unexpected detection of porcine rotavirus C strains carrying human origin VP6 gene

AU - Kattoor, Jobin Jose

AU - Saurabh, Sharad

AU - Malik, Yashpal Singh

AU - Sircar, Shubhankar

AU - Dhama, Kuldeep

AU - Ghosh, Souvik

AU - Bányai, K.

AU - Kobayashi, Nobumichi

AU - Singh, Raj Kumar

PY - 2017/12/1

Y1 - 2017/12/1

N2 - BACKGROUND: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog.OBJECTIVE: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes.METHODS: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed.RESULTS: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population.CONCLUSION: The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

AB - BACKGROUND: Rotavirus C (RVC), a known etiological agent of diarrheal outbreaks, mainly inflicts swine population globally with sporadic incidence in human, cattle, ferret, mink and dog.OBJECTIVE: To demonstrate the presence of RVC in Indian swine population and characterization of its selected structural (VP6) and non-structural (NSP4 and NSP5) genes.METHODS: A total of 108 diarrheic samples from different regions of India were used. Isolated RNA was loaded onto polyacrylamide gel to screen for the presence of RVs through the identification of specific electrophoretic genomic migration pattern. To characterize the RVC strains, VP6 gene and NSP4 and NSP5 genes were amplified, sequenced and analyzed.RESULTS: Based on VP6 gene specific diagnostic RT-PCR, the presence of RVC was confirmed in 12.0% (13/108) piglet fecal specimens. The nucleotide sequence analysis of VP6 gene, encoding inner capsid protein, from selected porcine RVC (PoRVC) strains revealed more than 93% homologies to human RVC strains (HuRVC) of Eurasian origin. These strains were distant from hitherto reported PoRVCs and clustered with HuRVCs, owning I2 genotype. However, the two non-structural genes, i.e. NSP4 and NSP5, of these strains were found to be of swine type, signifying a re-assortment event that has occurred in the Indian swine population.CONCLUSION: The findings indicate the presence of human-like RVC in Indian pigs and division of RVC clade with I2 genotype into further sub-clades. To the best of our knowledge, this appears to be the first report of RVC in Indian swine population. Incidence of human-like RVC VP6 gene in swine supports its subsequent zoonotic prospective.

KW - human origin

KW - I2 genotype

KW - Porcine

KW - rotavirus C

KW - swine

KW - VP6 gene

KW - zoonosis

UR - http://www.scopus.com/inward/record.url?scp=85037568761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037568761&partnerID=8YFLogxK

U2 - 10.1080/01652176.2017.1346849

DO - 10.1080/01652176.2017.1346849

M3 - Article

C2 - 28643555

AN - SCOPUS:85037568761

VL - 37

SP - 252

EP - 261

JO - Veterinary Quarterly

JF - Veterinary Quarterly

SN - 0165-2176

IS - 1

ER -