Under inflammatory stimuli mesenteric mesothelial cells transdifferentiate into macrophages and produce pro-inflammatory cytokine IL-6

Sándor Katz, Viktória Zsiros, A. Kiss

Research output: Contribution to journalArticle

Abstract

Objective: Inflammatory stimuli inducing epithelial-to-mesenchymal transition (EMT) can transdifferentiate mesenteric mesothelial cells into macrophages. Methods: Sprague Dawley rat mesenteric mesothelial cells were used as a model. 1 ml Freund adjuvant was injected into the peritoneal cavity of rat and GM-CSF treatment was used to induce inflammation. IL-10 and IL-6 expression were studied by immunocytochemistry and Western blot analysis both in vivo and in vitro. Results: Control mesothelial cell express anti-inflammatory IL-10, but no pro-inflammatory IL-6 expression could be detected in them. By the time of inflammation, IL-6 expression increased (reached the maximum level at the fifth day of inflammation), parallel to this the IL-10 entirely disappeared from these cells. In vitro GM-CSF treatment resulted in similar changes. As the mesothelial cells started to recover (at the eighth day of inflammation) IL-6 expression decreased and IL-10 level started to increase again. Conclusion: These data show that under inflammatory stimuli mesothelial cells—like macrophages—can produce pro-inflammatory cytokines.

Original languageEnglish
Pages (from-to)525-528
Number of pages4
JournalInflammation Research
Volume68
Issue number7
DOIs
Publication statusPublished - Jul 1 2019

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Interleukin-6
Interleukin-10
Macrophages
Cytokines
Inflammation
Granulocyte-Macrophage Colony-Stimulating Factor
Epithelial-Mesenchymal Transition
Freund's Adjuvant
Peritoneal Cavity
Sprague Dawley Rats
Anti-Inflammatory Agents
Western Blotting
Immunohistochemistry
Therapeutics
In Vitro Techniques

Keywords

  • Inflammation
  • Inflammatory cytokines
  • Mesothel/macrophage transition

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Under inflammatory stimuli mesenteric mesothelial cells transdifferentiate into macrophages and produce pro-inflammatory cytokine IL-6. / Katz, Sándor; Zsiros, Viktória; Kiss, A.

In: Inflammation Research, Vol. 68, No. 7, 01.07.2019, p. 525-528.

Research output: Contribution to journalArticle

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N2 - Objective: Inflammatory stimuli inducing epithelial-to-mesenchymal transition (EMT) can transdifferentiate mesenteric mesothelial cells into macrophages. Methods: Sprague Dawley rat mesenteric mesothelial cells were used as a model. 1 ml Freund adjuvant was injected into the peritoneal cavity of rat and GM-CSF treatment was used to induce inflammation. IL-10 and IL-6 expression were studied by immunocytochemistry and Western blot analysis both in vivo and in vitro. Results: Control mesothelial cell express anti-inflammatory IL-10, but no pro-inflammatory IL-6 expression could be detected in them. By the time of inflammation, IL-6 expression increased (reached the maximum level at the fifth day of inflammation), parallel to this the IL-10 entirely disappeared from these cells. In vitro GM-CSF treatment resulted in similar changes. As the mesothelial cells started to recover (at the eighth day of inflammation) IL-6 expression decreased and IL-10 level started to increase again. Conclusion: These data show that under inflammatory stimuli mesothelial cells—like macrophages—can produce pro-inflammatory cytokines.

AB - Objective: Inflammatory stimuli inducing epithelial-to-mesenchymal transition (EMT) can transdifferentiate mesenteric mesothelial cells into macrophages. Methods: Sprague Dawley rat mesenteric mesothelial cells were used as a model. 1 ml Freund adjuvant was injected into the peritoneal cavity of rat and GM-CSF treatment was used to induce inflammation. IL-10 and IL-6 expression were studied by immunocytochemistry and Western blot analysis both in vivo and in vitro. Results: Control mesothelial cell express anti-inflammatory IL-10, but no pro-inflammatory IL-6 expression could be detected in them. By the time of inflammation, IL-6 expression increased (reached the maximum level at the fifth day of inflammation), parallel to this the IL-10 entirely disappeared from these cells. In vitro GM-CSF treatment resulted in similar changes. As the mesothelial cells started to recover (at the eighth day of inflammation) IL-6 expression decreased and IL-10 level started to increase again. Conclusion: These data show that under inflammatory stimuli mesothelial cells—like macrophages—can produce pro-inflammatory cytokines.

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