Unaltered mRNA expression of calcitonin-like receptor and receptor activity modifying proteins in human arteries in stroke and myocardial infarction.

Karen Eskesen, J. Tajti, T. Hortobágyi, Szok Délia, L. Vécsei, Lars Edvinsson

Research output: Contribution to journalArticle

Abstract

Calcitonin-like receptor (CL-R) is a functional CGRP1-receptor when complexed with RAMP1 or an adrenomedullin-receptor or when complexed with RAMP2 or RAMP3. This study was carried out 1. to set up a method to examine the relative quantity of mRNA of CL-R, RAMP1, RAMP2 and RAMP3 in human coronary (CA), pulmonary (PA) and middle cerebral arteries (MCA), and 2. to examine the level of mRNA expression in cerebra- and cardiovascular diseases. The method was validated with respect to the use of postmortem tissue and we compared beta-actin and GAPDH as housekeeping genes. There was no time-dependent change in total RNA and level of mRNA for p-actin or GAPDH could be detected in vessels removed from 1 and 5 days post mortem. The expression of beta-actin appears lower in coronary artery than in pulmonary artery and middle cerebral artery with no significant difference for GAPDH; both worked well. There were some differences in mRNA expression for CL-R (higher) and RAMP3 (lower) in middle cerebral artery compared to coronary artery and pulmonary artery. There was no significant difference in mRNA for RAMP1 and RAMP2 in the three types of arteries. We did not observe any difference in mRNA for CL-R and RAMPs in arteries from patients with hemorrhagic stroke, arteriosclerosis and acute myocardial infarction when compared to patients without these diagnoses. Thus the mRNA expression seems to be unaltered in these disorders.

Original languageEnglish
Pages (from-to)459-466
Number of pages8
JournalIdeggyógyászati szemle
Volume60
Issue number11-12
Publication statusPublished - Nov 30 2007

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Receptor Activity-Modifying Proteins
Calcitonin Receptors
Arteries
Stroke
Myocardial Infarction
Messenger RNA
Middle Cerebral Artery
Actins
Pulmonary Artery
Coronary Vessels
Adrenomedullin Receptors
Arteriosclerosis
Essential Genes
Cerebrum
Cardiovascular Diseases
RNA
Lung

ASJC Scopus subject areas

  • Medicine(all)

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Unaltered mRNA expression of calcitonin-like receptor and receptor activity modifying proteins in human arteries in stroke and myocardial infarction. / Eskesen, Karen; Tajti, J.; Hortobágyi, T.; Délia, Szok; Vécsei, L.; Edvinsson, Lars.

In: Ideggyógyászati szemle, Vol. 60, No. 11-12, 30.11.2007, p. 459-466.

Research output: Contribution to journalArticle

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