Ultraweak light emission, free radicals, chilling and light sensitivity

É. Hideg, L. O. Bjorn

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Ultraweak light emission (UWLE) was measured from leaves of various chilling-sensitive (Lycopersicon esculentum, Cucumis sativus and Phaseolus vulgaris) and -tolerant (Pisum sativum and Vicia faba) plants after exposure to low (4-7°C) temperature in the light. UWLE increased upon chilling treatment combined with illumination with 200 μmol m-2 s-1 PAR in all plants, by about 30% in tolerant and by more than 100% in sensitive plants. It increased more when applied together with 400 μmol m-2 s-1 PAR: by 90-100% and by 250-280% in chilling-tolerant and -sensitive plants, respectively. Free radical production was detected by spin trapping EPR spectroscopy in thylakoid membranes isolated from the chilling-treated Lycopersicon esculentum and Vicia faba leaves. After 12 h chilling at 200 μmol m-2 s-1 PAR, free radical production was approximately 3 times greater in the former than in the latter species. The same ratio was approximately 6 if chilling was carried out at 400 μmol m-2 s-1 PAR, indicating the role of photooxidative stress in chilling injury. Our results also confirm that the stress-induced increase in UWLE is an indicator of free radical production and offers the possibility of using UWLE for monitoring the effect of chilling on temperature sensitive plants in an early stage.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalPhysiologia Plantarum
Volume98
Issue number2
DOIs
Publication statusPublished - 1996

Fingerprint

Photophobia
Free Radicals
Light
Vicia faba
Lycopersicon esculentum
Solanum lycopersicum var. lycopersicum
Spin Trapping
Cucumis sativus
Thylakoids
Temperature
Phaseolus
Peas
Lighting
Spectrum Analysis
chilling injury
cold treatment
thylakoids
Pisum sativum
Phaseolus vulgaris
lighting

Keywords

  • Chilling sensitivity
  • electron paramagnetic resonance
  • free radicals
  • light sensitivity
  • spin trapping
  • ultraweak light emission

ASJC Scopus subject areas

  • Plant Science

Cite this

Ultraweak light emission, free radicals, chilling and light sensitivity. / Hideg, É.; Bjorn, L. O.

In: Physiologia Plantarum, Vol. 98, No. 2, 1996, p. 223-228.

Research output: Contribution to journalArticle

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