Ultraviolet-B effects on Spirulina platensis cells: Modification of chromophore-protein interaction and energy transfer characteristics of phycobilisomes

S. Rajagopal, I. B. Jha, S. D.S. Murthy, P. Mohanty

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Exposure of ultraviolet-B (280-320 nm, 1.9 mW m-2 s-1) radiation of intact Spirulina platensis for 9 h caused specific loss of the 85.5 KDa anchor protein of phycobilisomes, the major light-harvesting antenna complex of photosystem II. Associated with the loss of 85.5 KDa protein, the UV-B irradiation also caused photobleaching of phycobilins and alteration in the chromophore protein interactions, as evidenced from the visible circular dichroic measurements, and it also affected the energy transfer process within the phycobilisomes, as inferred from the low-temperature, 77 K, fluorescence spectral analysis. Our results, thus, clearly demonstrate for the first time that the phycobilisomes effectively act as targets for UV-B induced damage of photosynthetic apparatus in cyanobacteria.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalBiochemical and biophysical research communications
Volume249
Issue number1
DOIs
Publication statusPublished - Aug 10 1998

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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